激光对荷瘤小鼠免疫系统影响的实验研究

Effect of Laser on IL-6,IL-8 and IL-1 in Mice with Tumor

目的借助于低能量的ＨｅＮｅ激光激活免疫机制，抑制肿瘤的生长。方法采用双抗体夹心ＥＬＩＳＡ法及ＭＴＴ法对荷瘤小鼠激光照射前后细胞因子（ＩＬ－１、ＩＬ－６、ＩＬ－８）的含量进行检测，并对其抑制肿瘤作用进行研究。结果激光照射瘤鼠脾区具有促进ＩＬ－１、ＩＬ－８产生，抑制ＩＬ－６及瘤细胞增殖的作用。与对照组相比有非常显著差异（Ｐ＜００１）。结论低能量激光照射具有调节患瘤鼠白细胞介素产生及抗肿瘤作用。

Objective To observe the effect of cyto laser on immulogical mechanism.Methods Double antibody sandwith enzyme linked immunosorbent assay(ELISA) and MIT test were used to measure the effect of laser on IL 6,IL 8 and IL 1 tumor bearing mice,Results When laser acted on spleen region,promoting IL 1 and IL 8 production and suppressing IL 6 production,compared with control group showed very significant changes ( P <0 01) Conclusion Laser has regulating effect of interleukin production and antitumor action