癌基因蛋白P^(21)、P^(185)、EGFR及LN、IV型胶原在骨肉瘤中的表达

Expression of p21、p185、EGFR、LN and Type IV Collagen in Human Osteosarcoma Tissue

目的探讨癌基因蛋白ｐ２１、ｐ１８５、ＥＧＦＲ及ＬＮ、ＩＶ型胶原在人骨肉瘤组织中表达的意义。方法应用免疫组化ＡＢＣ法，检测４０例常规石蜡包埋的骨肉瘤组织中ｐ２１、ｐ１８５和ＥＧＦＲ的表达，同时还检测了ＬＮ、ＩＶ型胶原在骨肉瘤组织中的含量。结果ｐ２１、ｐ１８５和ＥＧＦＲ在骨肉瘤组织中的阳性表达率分别为２６３％（１０／３８），６５８％（２５／３８），５００（１９／３８），同时观察到ＬＮ和ＩＶ型胶原在骨肉瘤组织中往往是缺如的。结论骨肉瘤组织中存在着多种癌基因的异常，它们与骨肉瘤的发生发展密切相关，同时细胞外基质的破坏在骨肉瘤的侵袭与转移中起着重要的作用。

Objective To evaluate the significance of p21,p185,EGFR protein expression and LN,type IV collagen in human osteosarcoma.Methods p21,p185,EGFR protein expression and LN,type IV collagen were examined in 40 cases human osteosarcoma tissue with immunohistochemical ABC methods.Results Accumulation of p21 protein was found in 8 out of 38 cases osteosarcomas.The positive rates of p185 and EGFR were respectively 65 8% and 50 0%.It was observed that the majority of LN and type IV collagen expression in osteosarcoma was defective.Conclusion The results of p21,p185 and EGFR high expression in human osteosarcoma suggest that they did have a correlation with osteosarcoma origin and development;The changes of LN and type IV collagen have significant effect on osteosarcoma invade and metastasis.