贵阳市412例腹泻病例空肠弯曲菌的检测分析

Detection and analysis of campylobacter jejuni in 412 diarrhea cases of Guiyang

目的建立常规的空肠弯曲菌检测方法,了解贵阳市腹泻人群空肠弯曲菌的感染状况。方法采用简单生化及多重PCR方法对贵阳市412例腹泻人群进行空肠弯曲菌检测。结果 412份腹泻标本中,7份空肠弯曲菌阳性,平均检出率1.70%。1岁以下婴幼儿感染率与其它人群差异无统计学意义(P>0.05)。男女患者感染率差异无统计学意义(P>0.05)。粘液便空肠弯曲菌检出率(7.69%)显著高于水样便(0.60%)(P<0.01)。结论利用简单生化及多重PCR方法能很好地检出空肠弯曲菌;贵阳市腹泻人群中存在空肠弯曲菌的感染,且主要感染婴幼儿,需加强婴幼儿腹泻中空肠弯曲菌感染的监测。

Objective To establish routine testing methods and understand campylobacter jejuni infection status in diarrhea patients in Guiyang.Method Using simple biochemical methods and multiple PCR methods to detect the campylobacter jejuni in 412 diarrhea patients in Guiyang city.Results 7 c jejuni strains were isolated from 412 diarrhea cases,and with the average detection rate of 1.70%.The infection rate of 1 year old had no significant difference with other crowds,the detection rate of men and female had no significant difference too.The detection rate of mucus stool（7.69%） was higher than watery stool（0.60%）（P0.01）.Conclusions Simple biochemical methods and multiple PCR methods were better for testing c jejuni.There were C jejuni infection in diarrhea patients in Guiyang city,and it mainly infected babies and infants,so we need to strengthen the monitoring of baby diarrhea.