蛋白质工程在提高蛋白质稳定性中的应用

The Application of Protein Engineering in Improving Protein Stability

过去18年中,通过蛋白质工程技术进行体外突变来提高蛋白质稳定性的大量文献被报道。这些报道涉及从利用最早的理性设计来处理像T4溶菌酶和芽孢杆菌RNA酶等一些小酶,以及目前的蛋白设计和定向进化技术。本文着重介绍提高蛋白质稳定性的途径以及通过理性设计、DNA改组、组合设计、数据驱动设计方法等提高蛋白质,特别是酶的稳定性的研究进展。

During the past 18 years there has been a continuous flow of reports describing proteins stabilized by the introduction of mutations.These reports span a period from pioneering rational design work on small enzymes such as T4 lysozyme and barnase to present protein design and directed evolution technique.This literature plays emphasis on introducing the paths to improve protein stability,and the advances of researches in enhance the stability of protein especially of the enzyme,by rational design method,DNA shuffling,combinational design and data-driven design methods.