CK19和TGF-α在大鼠肝纤维化部分肝切除后残肝中的表达

Expressions of CK19 and TGF-α in reserved liver after partial hepatectomy of rat with hepatic fibrosis

目的检测肝纤维化大鼠部分肝切除后不同时间点CK19及TGF-α的表达,了解其胆管再生情况。方法雄性SD大鼠,对照组和实验组各35只。实验组腹腔注射CCl4制备肝纤维化模型,两组均进行肝部分切除术。进行免疫组织化学、HE染色,图像分析和数据统计。结果验组和对照组术后随时间延长CK19表达均呈增强趋势;实验组术后各时间点CK19的表达均高于对照组同时间点。实验组和对照组术后随时间延长TGF-α表达均呈上升趋势,但实验组上升速度明显较对照组缓慢;实验组术后0d、1d、3d的TGF-α表达高于对照组。结论 (1)肝纤维化大鼠肝部分切除后CK19呈现高表达,提示肝纤维化部分切除可以促进肝卵圆细胞的增殖和分化。(2)TGF-α对肝纤维化大鼠肝部分切除后肝卵圆细胞增殖及胆管再生的促进作用不明显。

Objective To detect the expressions of CK19 and TGF-α in reserved liver after partial hepatectomy（PH） of rat with hepatic fibrosis at the different time phase,for evaluating the bile canaliculi regeneration in fibrotic liver tissue.Methods Male SD rats were divided into two groups： the control group（35 rats） and the experimental group（35 rats）.The rats of experimental group were injected i.p.with CCl4 to establish the hepatic fibrosis model,and then animals from two groups was endured PH operation.The liver tissues were obtained from each group at 0,1,3,5 and 7d after PH,followed by immunohistochemical analysis.Results（1） The expression of CK19 increased gradually after PH in both groups,however,in experimental group,the expression intensity was significantly higher than that of in control group at the same time point（P0.05） except for that at 7d.As well,the expression of TGF-α increased gradually after PH in both groups,however,The expression was significant higher in the experimental group at 0 d,1 d,3 d and lower at 5 d,7 d compared to that in the control group.Conclusions For rat model of hepatic fibrosis,the increased expression of CK19 can be detected in reserved liver after partial hepatectomy,which indicates that the proliferation and differentiation of HOCs can be promoted by hepatic fibrosis and PH treatment.In contrast,TGF-α is uneffective for promoting proliferation of HOCs and bile canaliculi regeneration.