盐酸戊乙奎醚对内毒素休克兔血清肿瘤坏死因子-α、一氧化氮、内皮素-1的影响

Effect of Penehyclidine Hydrochloride on Levels of TNF-α、NO、ET-1 in Serum in the Rabbit Model of Endotoxin Shock

目的:观察不同剂量盐酸戊乙奎醚(长托宁)对内毒素休克兔血清肿瘤坏死因子-α(TNF-α)、一氧化氮(NO)、内皮素-1(ET-1)浓度的影响。方法:35只健康新西兰大白兔随机分成正常对照组、休克组、长托宁低剂量组(0.05 mg/kg)、长托宁中剂量组(0.15 mg/kg)和长托宁高剂量组(0.45 mg/kg),每组7只。采用静脉注射大肠杆菌脂多糖制作内毒素休克模型。长托宁各组分别于静注脂多糖后立即静注不同剂量长托宁,测定给药后不同时间点动物血清TNF-α、NO、ET-1的含量。结果:休克组血清TNF-α、NO、ET-1浓度明显高于正常对照组(P<0.01);长托宁低、中、高剂量组血清TNF-α、NO、ET-1浓度较休克组均有不同程度减低(P<0.05),以中剂量组减低最明显(P<0.01或P<0.05)。结论:长托宁能部分抑制炎症介质的产生和释放,相对于低、高剂量长托宁,中剂量在抑制炎症介质方面效果更为显著。

Objective：To observe the effect of different doses of penehyclidine hydrochloride （PQN） on the levels of tumor necrosis faetor-α（TNF-α）, nitric oxide（NO）and endothelin-1 （ET-1）in serum in the rabbit model of endotoxin shock. Methods： 35 healthy white New Zealand rabbits were randomly divided into control group, shock group, low dose PQN group （0.05 mg/kg）, middle dose PQN group（0.15 mg/kg） and high dose PQN group（0.45 mg/kg）. Each group was 7.The endotoxin shock model was set up by the intravenous infusion with Lipopolysaccharide （LPS）.Coineident with the injection of LPS,the different doses of PQN were immediately administered in the low, middle, high dose PQN group, respectively. Assaying the levels of TNF-α,NO,ET-1 in serum of rabbits at the different time. Results： Compared with the control group, levels of TNF-α,NO,ET-1 in serum were decreased obviously in the shock group（P〈0.01）. Compared with the shock group, levels of TNF-α, NO, ET-1 in serum were decreased with vary degree in the PQN treatment groups（P〈0.05）, most obviously in the middle close PQN group（P〈 0.01 or P〈0.05）. Conclusions： PQN can decrease the levels of TNF-α, NO, ET-1 in serum in the rabbit model of endotoxin shock. Compared with the low and high dose of PQN, the middle dose of PQN has better significant effect.