阻塞性睡眠呼吸暂停低通气综合症患者外周血Th1/Th2细胞因子表达水平的研究

Th1/ Th2 Cytokines in Peripheral Blood Lymphocytes in Patients with Obstructive Sleep Apnea Hypopnea Syndrome

目的:检测阻塞性睡眠呼吸暂停低通气综合症(OSAHS)患者外周血CD_4^+T淋巴细胞分泌的细胞因子水平,探讨患者Th1/Th2相关细胞因子的平衡及其临床意义。方法:47例OSAHS患者按照呼吸暂停低通气指数(AHI)水平分为轻度组(n=16),中度组(n=17),重度组(n=14),18例健康人作为对照组,分别测定各组外周血中Th1细胞因子:干扰素γ(IFN-γ)、白细胞介素2(IL-2)和肿瘤坏死因子β(TNF-β);Th2的细胞因子:IL-4、IL-10和IL-12的含量。结果:OSAHS各组Th1型细胞因子中IFN-γ高于对照组,随着病情的进展有逐渐升高趋势,但仅在重度组与中度组、轻度组、健康组比较时P均<0.05,其余Th1/Th2细胞因子在进行两两比较时各组间均无明显差异。OSAHS患者IFN-γ与AHI呈正相关,而Th2细胞因子与AHI无相关关系。结论:OSAHS患者Th1/Th2平衡失调,向Th1方向漂移,Th1细胞表型处于优势状态,且与OSAHS的严重程度相关。

Objective： To detect the levels of Th1 and Th2 type cytokines of peripheral blood CD4^＋T lymphocytes in patients with obstructive sleep apnea hypopnea syndrome（OSAHS）and explore their clinical significance. Methods：Based on apnea hypopnea index（AHI） which measured by polysomnography （PSG）,47 patients with OSAHS were divided into three groups： mild group（n=16）,moderate group（n=17） and severe group and healthy people（n=18） as control group. The Th1 eytokines（IFN-γ, IL-2,TNF-β） and Th2 cytokines（IL-4,IL-10,IL-12） were measured. Results： The levels of Th1 type cytokines such as IFN-γ in patients with OSAHS were significantly higher than that in the control group,P〈0.05. The levels of Th1 positively correlated to AHI,P〈0.05.There were no significant difference of the other Th1/Th2 related cytokines （IL-2,TNF-β, IL-4, IL-10 and IL-12） between OSAHS and the control group. Conclusion： Th1/Th2 imbalances in OSAHS. IFN-γ is enhanced and Th1/Th2 shifts to Th1. It may be implicated in the pathogenesis of this disorder, and which is associated with the severity of OSAHS.