菟丝子提取物对大鼠心肌缺血/再灌注损伤的保护作用

Protective effects of Cuscuta chinensis Lam.extract on myocardial ischemia/reperfusion injury in rats

目的观察菟丝子提取物(CCLE)对大鼠心肌缺血/再灌注损伤(MI/RI)的保护作用。方法 30只SD大鼠随机分为3组(n=10):假手术组、生理盐水对照组、菟丝子提取物组。结扎左冠状动脉前降支,建立大鼠心肌缺血/再灌注损伤模型,记录各组心电图,HE染色观察心肌组织细胞形态,测定血清肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)、谷草转氨酶(AST)的含量变化。结果与对照组相比,CCLE可使MI/RI大鼠:①心率(HR)减慢(再灌注期各时间点P<0.05或P<0.01);②再灌注60、90、120 min时ST段明显降低(P<0.05或P<0.01);③心肌梗死程度明显减轻;④心肌酶CK、CK-MB、LDH、AST的含量(U.L-1)比对照组分别降低3 360(P<0.01)、5 478(P<0.01)、922(P<0.05)、235(P<0.01)。结论 CCLE对再灌注损伤心肌有明显的保护作用。

Aim To observe the protective effects of Cuscuta chinensis Lam.extract（CCLE）on myocardial ischemia/reperfusion injury in rats.Methods 30 SD rats were randomly divided into three groups（n=10 for each group）： Sham group,normal saline group（NS group）,and CCLE group.Ligation of the left anterior descending coronary artery was performed to establish the acute myocardial ischemia/reperfusion injury model in rats.Electrocardiogram（ECG）,morphology of myocardial histiocyte and the content of CK,CK-MB,LDH,AST in blood serum of each group are recorded,observed and determined respectively.Results Compared with NS group,CCLE could lead to：① Heart rate of MI/RI rats was slowed down（P0.05 or P0.01）.② Level of ST obviously declined when reperfused blood for 60,90,120 min（P0.05 or P0.01）.③ The degree of myocardial infarction was clearly reduced.④ The content（U·L-1） of CK,CK-MB,LDH,AST in blood serum was reduced by 3 360（P0.01）,5 478（P0.01）,922（P0.05）,235（P0.01） respectively compared with the NS group.Conclusion CCLE has a significant protective effect on myocardial ischemia/reperfusion injury in rats.