检测乳腺癌HER-2基因扩增与HER-2蛋白表达的相关性

Correlation of detecting HER-2 gene amplification and HER-2 protein expression in breast cancer

目的探讨荧光原位杂交(FISH)法检测乳腺癌HER-2基因扩增与免疫组化(IHC)法检测HER-2蛋白表达结果的相关性。方法采用FISH和IHC方法分别检测134例乳腺癌石蜡标本的HER-2基因扩增和HER-2蛋白表达。结果 45例HER-2蛋白表达为(-)的病例中,HER-2基因扩增均为阴性;33例HER-2蛋白表达为(+)的病例中,4例HER-2基因扩增阳性;44例HER-2蛋白表达为()的病例中,28例HER-2基因扩增阳性;12例HER-2蛋白表达为()的病例中,11例HER-2基因扩增阳性。FISH与IHC检测结果的总符合率为84.3%,两者呈显著相关(P<0.01)。结论 IHC可作为筛查乳腺癌患者HER-2的首选方法。当HER-2蛋白表达为(-)或()时,IHC和FISH检测的一致性较高;而对于IHC为(+)或()的患者应再进行FISH检测,以确定HER-2基因表达。

Objective To investigate the correlation of HER-2 gene amplification reported by fluorescence in situ hybridization（FISH） and HER-2 protein expression detected by immunohis-tochemistry（IHC） in breast cancer.Methods HER-2 gene amplification and HER-2 protein expression in paraffin-embedded tissues specimens from 134 patients with breast cancer were examined with FISH and IHC,respectively,and the correlation between the results reported by two techniques were analyzed.Results All of 45 cases with negative HER-2 protein expression were negative in HER-2 gene amplification.Of 33 patients with HER-2 protein expression（＋）,four cases showed positive HER-2 gene amplification.Of 44 patients with HER-2 protein expression（）,28 cases showed positive HER-2 gene amplification.Of 12 patients with HER-2 protein expression（）,11 cases showed positive HER-2 gene amplification.The results reported by two methods were significantly correlated with a total accordance rate of 84.3%（P0.01）.Conclusion IHC can be used as a method for screening HER-2 expression status in breast cancer.A high accordance exists between the result reported by IHC and that by FISH when HER-2 protein expression is（-） or（）,whereas the patients with HER-2 protein expression（＋） or （） by IHC should undergo FISH test further in order to conferm HER-2 gene expression status.