摘要
EMS30是拟南芥经甲基磺酸乙酯(EMS)诱变得到的白化突变体。该突变体的叶绿体结构存在严重缺陷,同时伴随叶绿素缺失。遗传分析显示EMS30突变体的突变表型受隐性单基因控制。采用图位克隆的方法对EMS30突变基因进行定位的结果显示,该基因位于拟南芥第一条染色体的分子标记F21M12和F14N23之间的96kb区间内,该区间包含25个基因。通过生物信息学分析发现,该区间内有3个基因定位在叶绿体或与叶绿体发育相关。这些结果有助于该基因的克隆,为阐释叶绿体发育提供线索。
An Arabidopsis thaliana albino mutant EMS30 was obtained by ethyl methane sulphonate （EMS） mutagenesis strategy. EMS30 showed severe defects in chloroplast ultrastructure in concomitance with chlorophyll deficiency. Genetic analysis indicated that albino phenotype was controlled by a single recessive locus. Using map-based cloning technique, EMS30 has been mapped to a region of 96 kb between molecular markers F21M12 and F14N23 on chromosome 1. In this region there are 25 genes, of which 3 genes are related with chloroplast function by bioinformatic analysis.
出处
《植物生理学报》
CAS
CSCD
北大核心
2011年第3期263-268,共6页
Plant Physiology Journal
基金
国家“973”项目(2009CB118504)
关键词
拟南芥
白化
图位克隆
Arabidopsis thaliana
albino
map-based cloning
