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马身猪和大白猪不同组织DECR1基因的表达 被引量：4

Expression Analysis of DECR1 Gene in Tissues of Mashen and Large White Pig
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摘要 旨在研究DECR1基因在猪不同组织和品种中mRNA和蛋白水平的表达规律,探讨该基因与脂肪代谢的关系。以山西马身猪与大白猪为试验材料,提取肝脏、心脏、肾脏、脾脏、肺脏、胃、小肠、皮下脂肪和背最长肌组织的总RNA和总蛋白,应用实时荧光定量PCR技术检测DECR1基因在2个品种各组织中mRNA的相对表达量,采用Western blot技术对各组织中DECR1蛋白进行半定量分析。实时荧光定量PCR结果显示:DECR1基因在各组织中均有表达,组织之间的表达量存在极显著差异(P<0.01),DECR1基因在肝脏、皮下脂肪与心脏中为高丰度表达;在不同品种的皮下脂肪组织中,大白猪DECR1的mRNA表达极显著高于马身猪(P<0.01)。Western blot检测结果显示:DECR1在各组织中均有表达,不同组织的表达量存在极显著差异(P<0.01),在皮下脂肪、肝脏与小肠中高表达;不同品种的皮下脂肪组织中,大白猪DECR1蛋白的表达显著高于马身猪(P<0.05)。猪DECR1基因在不同组织的表达差异可能与脂肪代谢和脂肪沉积有关。 The purpose of this study was to analyze DECR1 mRNA and protein expression profiles of Mashen and Large White pigs in different tissues,and evaluate the potential relationships between its relative expression and lipid metabolism.DECR1 expression profiles in nine tissues（liver,kidney,spleen,lung,small intestine,subcutaneous adipose,stomach,longissimus dorsi,heart） of Mashen and Large White pigs were examined by quantitative real-time PCR and Western blot,respectively.The results indicated that,by real-time PCR,DECR1 had the tissue-and species-specific in mRNA expression,but highly in liver,subcutaneous adipose and heart.The DECR1 mRNA expression in subcutaneous adipose of Large White pig was significantly higher than that of Mashen pig（P0.01）.The results showed that,by Western blot,the distribution of DECR1 in tissues were significantly different,but highly in subcutaneous adipose,liver and small intestine.Moreover,the expression of DECR1 in subcutaneous adipose of Large White pig was significantly higher than that of Mashen pig（P0.05）.It was inferred that DECR1 gene may be related to regulatory mechanism of lipid metabolism.
作者 李步高 郭晓红 曹果清 王效京 高鹏飞 刘宏 周忠孝
机构地区 山西农业大学动物科技学院 山西省畜禽繁育工作站 大同市种猪场
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2011年第4期475-480,共6页 ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金 山西省科技攻关项目(021043 20080311031) 国家农业科技成果转化项目(2008GB2A300032)
关键词 猪 DECR1基因 实时荧光定量PCR WESTERN BLOT 组织表达 pig DECR1 gene quantitative real-time PCR Western blot tissue expression
分类号 S828 [农业科学—畜牧学] Q344 [生物学—遗传学]
  • 相关文献

参考文献18

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二级参考文献30

  • 1KUNAU W H, DOMMES P. Degradation of unsaturated fatty acids. Identification of intermediates in the degradation of cis-4-decenoly-CoA by extracts of beefliver mitochondria [J]. Eur J Biochem, 1978, 91: 533-544.
  • 2YOU S, COSLOY S, SCHULZ H. Evidence for the essential function of 2, 4 -Dienoyl -CoA reductase in the β- oxidation of unsaturated fatty acids in vivo [J]. JBiolChem, 1989, 264: 16489-16495.
  • 3ROE C R, MILLINGTON D S, NORWOOD D L, et al. 2, 4-Dienoyl -coenzyme A reductase deficiency: a possible new disorder of fatty acid oxidation[J]. J Clin Invest, 1990, 85(5):1703-1707.
  • 4HELANDER H M, KOIVURANTA K T, HORELLI-KUITUNEN N, et al. Molecular cloning and characterization of the human mitochondrial 2, 4- dienoyl- CoA reductase gene (DECR) [J]. Genomics, 1997, 46: 112-119.
  • 5CLOP A, CERCOS A, TOMAS A, et al. Assignment of the 2, 4-dienoyl-CoA reductase (DECR) gene to porcine chromosome 4 [J]. Anita Genet, 2002,33: 164-165.
  • 6PEREZ-ENCISO M, CLOP A, NOGUERA J L, et al. A QTL on pig chromosome 4 affects fatty acid metabolism: evidence from an Iberian by Landrace intercross [J]. J Anim Sci, 2000, 78: 2525-2531.
  • 7CLOP A, OVILO C, PEREZ-ENCISO M, et al. Detection of QTL affecting fatty acid composition in the pig [J]. Mamm Genome, 200a, 14: 650-656.
  • 8DAVOLI R, FONTANESI L, ZAMBONELLI P, et al. Isolation of porcine expressed sequence tags for the construction of a first genomic transcript map of the skeletal muscle in pig [J]. Anita Genet, 2002, 33 : 3-18.
  • 9AMILLS M, VIDAL O, VARONA L, et al. Polymorphism of the pig 2, 4 -dienoyl CoA reductase 1 gene (DECR1) and its association with carcass and meat quality traits [J]. J Anita Sci, 2005, 83: 493- 498.
  • 10WALLING G A, VISSCHER P M, ANDERSSON L, et al. Combined analyses of data from quantitative trait loci mapping studies. Chromosome 4 effects on porcine growth and fatness [J]. Genetics, 2000, 155 : 1369-1378.

共引文献4

同被引文献43

  • 1解用虹.不饱和脂肪酸的β氧化[J].生命的化学,1994,14(2):46-48. 被引量：2
  • 2Kunau W H, Dommes P. Degradation of unsaturated fatty acids. I- dentification of intermediates in the degradation of cis-4-decenoyl- CoA by extracts of beefliver mitochondria [J]. Eur J Biochem, 1978, 91: 533-544.
  • 3Helander H M, Koivuranta K T, Horelli-Kuitunem N, et cd. Molecular cloning and characterization of human 2,4-dienoyl- CoA reduetase 1 ~eue[J]. Genomies, 1997, 46: 112-119.
  • 4Roe C R, Millington D S, Norwood D L, et oi. 2, 4-Dienoyl - coenzyme A reductase deficiency: a possible new disorder of fatty acid oxidation [J]. J Clin Invest, 1990, 85(5): 1 703-1 707.
  • 5Clop A, Ovilo C, Perez Enciso M, et aL Detection of QTL affect- ing fatty acid composition in the pig[J]. Mamm Genome, 2003, 14: 650-656.
  • 6Perez-Enciso M, Clop A, Noguera J L, et al. A QTL on pig chro- mosome 4 affects fatty acid metabolism: evidence from an Iberian by Landrace intercross [J]. J Anita Sci, 2000, 78:2 525-2 531.
  • 7Walling G A, Visscher P M, Andersson L, et al. Combined analy- ses of data from quantitative trait loci mapping studies. Chromo- some 4 effects on porcine growth and fatness[J]. Genetics, 2000, 155: 1369-1378.
  • 8Kaminski S, Brym P, Wojcik E. A note on associations between polymorphism within the 2, g-dienoyl-CoA reductase gene (I)E- CR1) and growth rate of Polish Landrace boars[J]. J Anita Feed Sci, 2009, 18: 71-77.
  • 9Davoli R, Fontanesi L, Braglia S, et d. A missense mutation in the porcine mitochondrial 2, 4-dieoyl CoA reductase 1 (DECR1) gene and linkage mapping of this locus to chromosome 4 [J]. Anim Genet, 2002, 33: 72-84.
  • 10Amills M, Vidal O, Varona L, et al. Polymorphism of DECRI and its association with carcass and meat quality traits[J]. J Anita Sci, 2005, 83: 493-498.

引证文献4

二级引证文献33

畜牧兽医学报
2011年 第4期

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