3种禽类呼肠孤病毒血清学相关性及致细胞病变差异分析

The Comparison of Serologic Relativity and CPE Types of 3 Avian Reovirus Strains Induced Different Diseases

本研究旨在研究3种不同疾病型禽类呼肠孤病毒间的抗原性关系及病毒的培养特性。作者通过血清中和试验测定了禽呼肠孤病毒(ARV S1133株)、番鸭呼肠孤病毒(MDRV 9710株)、新型鸭呼肠孤病毒(NDRV NP01株)3种禽类呼肠孤病毒的血清学相关性,统计抗原相关性R值;并应用部分禽胚原代细胞及哺乳动物传代细胞对这3种病毒的培养特性进行了初步研究。结果表明,3种病毒株之间的R值很小,抗原相关性较低;三者具有广泛的细胞亲嗜性,能在多种细胞中增殖,并产生细胞病变,但病毒致细胞病变特征有所差异,ARV和NDRV均以巨融合为主,而MDRV则以细胞圆缩坏死为主。上述结果表明导致禽类不同疾病的ARV、MDRV和NDRV三者之间的抗原相关性较低,病毒的细胞培养特性也不同,细胞病变类型的差别提供了一种初步鉴别禽类呼肠孤病毒的方法。

This study was conducted to compare the antigenic relativity and cytopathic effect（CPE） characteristics of 3 avian reovirus isolates induced different diseases.The serologic relatedness of avian reovirus（ARV） S1133 strain,muscovyduck reovirus（MDRV） MW9710 strain,novel duck reovirus（NDRV） NP01 strain were determined using cross virus-neutralization（VN） tests.Two avian and five mammalian cell lines were evaluated for their application in propagating these avian reovirus strains.Relatedness（R） values,determined by cross VN,revealed that three isolates＇ antigen had major differences.These strains could be cultivated successfully in Vero,MDEK,CEF,MDEF,ST,AD293 T cell lines and make some different types of CPE to different cell lines.These results indicated that ARV,MDRV and NDRV＇ antigen had major differences and did not share same antigenic relationship with each other.Avain reovirus of different disease-type could be distinguished by the types of CPE.