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胸膜肺炎放线杆菌菌影疫苗免疫仔猪前后差异表达基因的鉴定与分析 被引量：1

Identification and analysis of differential expression genes in peripheral blood lymphocytes from piglet immunized by bacterial ghost of Actinobacillus pleuropneumoniae
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摘要 为获得胸膜肺炎放线杆菌(APP)菌影诱导的仔猪淋巴细胞差异表达基因,本研究应用代表性差异分析技术构建APP菌影免疫前后正、反两个外周血淋巴细胞cDNA差减文库,并对文库中的差异基因进行克隆、测序和生物信息学分析。试验结果表明,正向文库中获得11个表达丰度上调的基因,其中7个基因与已知基因具有相似性,4个为未知新基因,经进一步功能注解发现,正向文库功能基因包括免疫信号传导相关蛋白RhoE、防御相关蛋白糖基转移样酶-1、上皮膜蛋白2、白介素-17和肿瘤免疫相关的周期素依赖性蛋白激酶抑制因子3等,这些功能基因表达丰度升高,可能有助于机体建立抗APP的免疫应答。 To screen differential expression genes in peripheral blood lymphocytes induced by ghost of Actinobacillus pleuropneumoniae, the forward and reverse two subtractive cDNA libraries were constructed from the peripheral blood lymphocytes of piglet vaccinated by bacterial ghost of A. pleuropneumoniae using representational difference analysis technique. The analysis identified differentially expressed transcripts. The results indicated that genes related to immunization signal transduction, disease defence related protein, epithelial membrane protein and interleukin-17, tumor immunity related factors were up-regulated after vaccinated, which may increase the immunity response.
作者 杨舒心 雷连成 杜崇涛 王瑜 谢芳 韩文瑜
机构地区 吉林大学畜牧兽医学院 中国农业科学院哈尔滨兽医研究所
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2011年第4期276-280,共5页 Chinese Journal of Preventive Veterinary Medicine
基金 国家自然科学基金项目(31072133)
关键词 胸膜肺炎放线杆菌 外周血淋巴细胞 代表性差异分析 差异表达基因 Actinobacillus pleuropneumoniae peripheral blood lymphocyte representational difference analysis differential expression genes
分类号 S852.61 [农业科学—基础兽医学]
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参考文献11

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同被引文献26

  • 1刘东胜,朱必凤,杨旭夫.多杀性巴氏杆菌幽灵的研究[J].韶关学院学报,2008,29(12):79-83. 被引量：4
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  • 3Yu SY, Peng W, Si W, et al. Enhancement of baeteriolysis of shuffled phage PhiX174 gene E[J]. Virol J,2011,8:206.
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  • 5Wen J ,Yang Y,Zhao G,et al. Salmonella typhi Ty21a bacteri- al ghost vector augments HIV-1 gp140 DNA vaccine-induced peripheral and mucosal antibody responses via TLR4 pathway[J]. Vaccine,2012,30(39) : 5733 5739.
  • 6Cai K, Zhang Y, Yang B, et al. Yersinia enterocolitica ghost with msbB mutation provides protection and reduces proinflam- matory cytokines in rn{ee[J]. Vaccine,2013,31(2) :334-340.
  • 7Cai K, Gao X, Li T, et al. Intragastric immunization of mice with enterohemorrhagie Escherichia coli O157:H7 bacterial ghosts reduces mortality and shedding and induces a Th2-type dominated mixed immune response[J]. Can J Microbiol, 2010, 56(5) :389-398.
  • 8Kudela P, Koller VJ, Lubitz W. Bacterial ghosts (BGs)-ad- vanced antigen and drug delivery system[J]. Vaccine, 2010,28 (36) :5760-5767.
  • 9Tuntufye H N, Ons E, Pham A D, et al. Escherichia coli ghosts or live E. con expressing the ferri-siderophore recep tors FepA, FhuE, Iron and lutA do not protect broiler chickens against avian pathogenic E. coli (APEC)[J]. Vet Microbiol,2012,159(3-4) :470-478.
  • 10Katinger A, I.ubitz W,Szostak M P, et al. Pigs aerogenously immunized with genetically inactivated (ghosts) or irradiated Actinobacillus pleuropneumoniae are protected against a ho- mologous aerosol challenge despite differing in pulmonary eel lular and antibody responses[J]. J Biotechnol, 1999,73 (2-3) : 251-260.

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中国预防兽医学报
2011年 第4期

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