摘要
目的:观察胰岛素腹腔和皮下给药对2型糖尿病模型(KK小鼠)血脂的影响。方法:选择空腹血糖高于20 mmol/mL的KK小鼠作为2型糖尿病模型,分为腹腔给药组(腹腔给药组,n=9)和皮下给药组(皮下给药组,n=9)。C57BL小鼠作为正常对照(正常对照组,n=10)。2型糖尿病KK小鼠连续给予高糖高脂饮食4周,同时IP组每日一次注射胰岛素0.3 mL,皮下给药组每日一次注射胰岛素0.4 mL(速效胰岛素?精蛋白锌胰岛素=2?1),以维持空腹血糖正常(6.0±1.5)mmol/L。结果:胰岛素治疗2~4周,腹腔胰岛素给药可使2型糖尿病KK小鼠血清TG、TC、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)的浓度达到正常水平。皮下胰岛素给药只能使TC达到正常水平。结论:与胰岛素皮下给药相比,腹腔胰岛素给药能够更有效地纠正2型糖尿病KK小鼠脂代谢异常。
AIM: To study the effect of intraperitoneal and subcutaneous insulin administration on serum lipids in a type 2 diabetic animal model(KK mice). METHODS: KK mice with fasting blood sugar level higher than 20 mmol/mL were set as type 2 diabetic model and divided into subcutaneous injection group(s.c.group,n=9) and introperitoneal injection group(i.p.group,n=9).C57BL mice were set as normal control(C group,n=10).The type 2 diabetic KK mice were given high glucose and fatty for 4 weeks.Meanwhile,insulin was administrated at doses of 0.4 mL/d for s.c.group and 0.3 mL/d for i.p.group to normalize fasting blood glucose concentration(6.0 mmol/L±1.5 mmol/L).RESULTS:After the diabetic KK mice were treated with insulin for 2-4 weeks,the serum TG,TC,LDL-C and HDL-C were recovered to the normal scope by intraperitoneal injections of insulin,but TG,LDL-C and HDL-C were not recovered to the normal level by subcutaneous injections of insulin.CONCLUSION: This result indicates that compared with subcutaneous insulin administration,intraperitoneal insulin administration is more efficacious to correct the disorder of lipid metabolism.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2011年第2期130-134,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
天津市自然科学应用技术重点资助项目(08JCZDJC16000)
关键词
糖尿病
胰岛素
脂代谢
Diabetes mellitus
Insulin
Lipid metabolism