摘要
为探讨共刺激分子B7-H4在牛Ⅱ型胶原(CⅡ)诱导的类风湿关节炎(CIA)模型小鼠免疫器官、关节及RA患者滑膜组织内的表达,使用牛Ⅱ型胶原免疫DBA-1/j小鼠,建立小鼠类风湿关节炎模型。免疫组化检测B7-H4的表达变化,结果证实小鼠的胸腺、脾脏及淋巴结中有大量的B7-H4阳性细胞,免疫荧光分析结果表明这些B7-H4+细胞主要为CD31+内皮细胞。统计分析表明,CIA小鼠体内免疫器官中B7-H4表达及分布相对于对照组小鼠没有显著提高,但是RA患者及CIA小鼠的滑膜组织内发现大量B7-H4阳性细胞。鉴于关节炎滑膜组织内有大量B7-H4阳性细胞,提示其有可能参与并调节了关节炎的病理进程。
The expression and distribution of B7-H4 protein in tissues from bovine collagen II（CII）-induced mouse rheumatoid arthritis（CIA） and synovial tissues of RA patients was detected and analyzed by immunohistochemistry,in which the fluorescence double-staining was further used to characterize B7-H4 expression.Immunohistochemical analysis revealed that B7-H4 positive cells were found in thymus,spleen,lymph nodes and bone marrow,and fluorescence double-staining further demonstrated that B7-H4 positive cells were CD31＋ endothelial cells.However,the expression and distribution of B7-H4 was not changed significantly between normal and CIA mice.Interestingly,high level of B7-H4 positive cells was found both in synovial tissues of CIA mice and RA patients.The expression of B7-H4 was observed on the surface of antigen presentation cells,such as endothelial cells within immune organs and synovial tissues of CIA mice and RA patients,indicated this signal might be involved in regulating local T cell activation and finally participated in the pathogenesis of RA.
出处
《现代免疫学》
CAS
CSCD
北大核心
2011年第2期130-134,共5页
Current Immunology
基金
国家自然科学基金资助项目(30700855
60971117)
