摘要
目的研究烟雾暴露对大鼠肺泡巨噬细胞(AM)曲霉防御功能的影响。方法建立烟雾暴露大鼠模型,分离肺泡巨噬细胞,并用免疫荧光法进行鉴定。加入烟曲霉孢子共培养,测定AM的吞噬率及吞噬指数;用ELISA方法检测培养上清中TNF-α和IL-1β的浓度;用免疫组化检测树突状细胞相关性C型植物血凝素-1(Dectin-1)蛋白的表达。结果烟雾暴露组AM对烟曲霉孢子的吞噬率下降(P<0.05),吞噬指数无显著变化。孢子刺激后,烟雾暴露组培养上清中TNF-α和IL-1β的浓度显著低于对照组(P<0.05)。烟雾暴露组AM中Dectin-1蛋白的表达低于对照组(P<0.05)。结论烟雾暴露使大鼠AM吞噬能力下降,对曲霉的防御功能受损,这种功能变化可能与Dectin-1蛋白表达降低有关。
To investigate the effects of smoke exposure on defense function of alveolar macrophage（AM） against Aspergillus in rat,we set up a rat model of smoke exposure with normal rat as controls.Alveolar macrophages of model rat were isolated and identified by immunofluorescence,then co-cultured with Aspergillus fumigatus conidia to measure the phagocytic rate and phagocytic index.The concentration of TNF-α and IL-1β in the culture supernatant was evaluated by ELISA.The expression of dendritic cell-associated C-type lectin-1（Dectin-1） protein was detected by immunohistochemistry.We found that macrophage phagocytic rate of Aspergillus fumigatus conidia decreased significantly（P 0.05） in smoke exposure group,but no significant difference was observed on phagocytic index between two groups.After stimulation with conidia,ELISA showed that the concentration of TNF-α and IL-1β in culture supernatant of smoke exposure group was significantly lower than that in control group（P 0.05）.While immunohistochemistry indicated the expression of Dectin-1 protein in smoke exposure group was less than that in control group（P 0.05）.In conclusion,smoke exposure can impair the defense function of AM to Aspergillus,and this change may be associated with decreased expression of Dectin-1 protein in AM.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2011年第3期207-210,共4页
Immunological Journal
