光枝勾儿茶提取物的抗氧化活性

Evaluation on antioxidant activities of extracts from Berchemia polyphylla var. leioclada

目的探讨光枝勾儿茶提取物的抗氧化活性。方法采用80%乙醇进行回流提取,其中醇提浸膏依次用石油醚、乙酸乙酯、正丁醇萃取。分别考察光枝勾儿茶各提取物对羟基自由基(.OH)、二苯代苦味基肼自由基(DPPH.)的清除率以及对Fe3+的还原力。结果与抗坏血酸(Vc)比较,光枝勾儿茶提取物对自由基均有较强清除作用,对.OH的清除率Vc>乙酸乙酯萃取物>石油醚萃取物>正丁醇萃取物>水层部分,半清除率浓度(IC50,mg/ml)分别为0.061、0.099、0.344、0.770、1.709;对DPPH.的清除率Vc>正丁醇萃取物>乙酸乙酯萃取物>水层部分>石油醚萃取物,其半清除率浓度(IC50,mg/ml)分别为0.004 6、0.011 1、0.019 2、0.028 3、0.058 6。不同提取物对Fe3+有较强还原能力,还原力大小为:Vc>乙酸乙酯萃取物>正丁醇萃取物>石油醚萃取物>水层部分。结论光枝勾儿茶具有明显的抗氧化活性,在抗衰老方面有广泛应用前景。

Objective To study antioxidant activities of extracts from Berchemia polyphylla var.leioclada in vitro.Methods The powder of B.polyphylla was treated with 80% ethanol.The ethanol extract was extracted respectively using petroleum ether,ethyl acetate,n-butanol.Hydroxyl free radical（·OH）,DPPH free radical（DPPH·） and deoxidization Fe3＋ reaction system were used to observe in vitro antioxidant effects of the above extracts.Results Compared with the ascorbic acid（Vc）,the extracts had strong scavenging effect on free radicals,the activity sequence for scavenging ·OH was Vc ethyl acetate extract petroleum ether extract n-butanol extract water layer extract,which corresponding IC50 values were 0.061,0.099,0.344,0.770 and 1.709 mg/ml respectively;The capability of scavenging DPPH was Vc n-butanol extract ethyl acetate extract water layer extract petroleum ether extract,and the corresponding IC50 values were 0.004 6,0.011 1,0.019 2,0.028 3,0.058 6 mg/ml respectively.Different extracts have strong deoxidization ability of Fe3＋,the activity sequence was Vc ethyl acetate extract n-butanol extract petroleum ether extract water layer extract.Conclusions B.polyphylla has the stronger antioxidative activities and will be widely applied on antiaging.