天蚕壮阳散对中老年男性雄激素部分缺乏综合征模型大鼠睾丸间质细胞stAR蛋白表达的影响

Effect of Tiancan Zhuangyang Powder on the stAR Protein Expression of Leydig Cells in Model Rats with Partial Androgen Deficiency of Agine Male

目的观察天蚕壮阳散对中老年男性雄激素部分缺乏综合征(partial androgen deficiency of agingmale,PADAM)模型大鼠睾丸间质细胞中类固醇合成快速调节蛋白(steroidogenic acute regulatory protein,stAR蛋白)表达的影响。方法以环磷酰胺造成生殖系统受损、雄激素部分缺乏的PADAM大鼠模型,按照随机分组的原则分为4组:正常对照组、模型组、丙酸睾酮组、天蚕壮阳散组,观察各组大鼠一般情况、体重、悬尾实验和强迫游泳实验、睾丸指数等改变以评价大鼠自身状况,放射免疫法测定各组治疗前后血清总睾酮(total testosterone,TT)、游离睾酮(free testosterone,FT)水平,免疫组化法测定各组大鼠睾丸间质细胞stAR蛋白表达水平,进行统计分析。结果经环磷酰胺造模后,大鼠血清TT、FT水平下降(P<0.01)、行为学(悬尾试验)部分改变,基本类似PADAM,说明造模成功。两治疗组经治疗后血清TT、FT的含量较治疗前及模型组治疗后均显著提高(P<0.01),两治疗组比较差异无统计学意义(P>0.05)。两治疗组经治疗后悬尾实验不动时间均显著缩短(P<0.05),力竭游泳实验时间与模型组比较显著延长(P<0.05)。stAR蛋白灰度值显著下降(P<0.01)。结论天蚕壮阳散可阻止PADAM模型大鼠血清TT、FT的下降,提高stAR蛋白活性,改善抑郁状态和肌张力,其作用与丙酸睾酮相当。

Objective To observe the effect of Tiancan Zhuangyang Powder（TCZYP） on the steroidogenic acute regulatory protein（stAR protein） expression of Leydig cells in model rats with partial androgen deficiency of aging male（PADAM）.Methods PADAM rat model was prepared by cyclophosphamide induced reproductive system damage.Rats were randomly divided into four groups,i.e.the normal control group,the model group,the testosterone propionate group,and the TCZYP group.The general condition,body weight,tail suspension experiment and exhaustion swimming test,and the testicular index,etc.were observed to assess the state of rats.The serum total testosterone（TT）,and free testosterone（FT） levels were detected by radioimmunoassay before and after treatment.The stAR protein expression level of Leydig cells were determined using immunohistochemical assay.Statistic analyses were performed.Results By modeling with cyclophosphamide,serum TT and FT levels decreased（P0.01）,behavior changes were basically similar to PADAM（by tail suspension test）,indicating a successful modeling.After treatment serum TT and FT levels in the testosterone propionate group and the TCZYP group significantly increased when compared with before treatment and with the model group after treatment（P0.01）.There was no statistical difference between the TCZYP group and the testosterone propionate group（P0.05）.The immobility time in the taiI suspension test were significantly shortened in the testosterone propionate group and the TCZYP group（P0.05）.The exhausted swimming time was more significantly prolongated than that of the model group（P0.05）.The stAR protein gray value significantly decreased（P0.01）.Conclusion TCZYP could prevent serum TT and FT levels from decrease,improve stAR protein activities,and attenuate the depression state and muscular tension in PADAM rats.Its action was equivalent to that of testosterone propionate.