淋巴细胞中POT1 mRNA表达、端粒酶活性与2型糖尿病发生发展的关系

Relationship of POT1 mRNA expression,telomerase activity and development of type 2 diabetes

目的:研究淋巴细胞中端粒保护蛋白(POT1)的表达、端粒酶活性与2型糖尿病发生发展的关系。方法:以2型糖尿病患者162例为实验组,以健康体检者162例为对照组,RT-PCR检测POT1mRNA的表达;TRAP-ELISA分析端粒酶活性。结果:实验组POT1 mRNA的表达和端粒酶活性均明显高于对照组。其中血糖控制尚可组和差组分别与对照组比较,POT1 mRNA表达和端粒酶活性均明显增高;按糖尿病性视网膜病变分期中各组POT1mRNA表达和端粒酶活性与对照组比较存在显著性差异;且病程5～10年组、10年以上组与5年以下组相比,POT1mRNA表达和端粒酶活性也存在显著性差异。结论:2型糖尿病淋巴细胞中POT1mRNA的表达和端粒酶活性均明显增高,且与病情发展呈正相关。POT1和端粒酶的表达变化作为预后的评价依据有重要的临床意义。

Objective：To study the relationship of protection of telomeres 1（POT1） mRNA expression,telomerase activity in lymphocytes with the development of type 2 diabetic mellitus（T2DM）.Methods：One hundred and sixty-two cases with T2DM were test group,162 cases of healthy were control group,real-time quantitative PCR was used to analysis POT1 mRNA expression,semi-quantitative TRAP-ELISA was used to analysis telomerase activity.Results：The expression of POT1 mRNA and telomerase relatively activity in test group were obviously higher than those in control group（P 0.01）.Meanwhile,the POT1 mRNA expression and telomerase activity were significantly higher in T2DM patients with passable or poor controlled blood glucose compared with controls（P 0.01）,and they were also significantly higher in T2DM patients with different stage of diabetic retinopathy compared with controls（P 0.01）,significant differences also existed among the patients whose time course of T2DM between 5 to 10 years or above 10 years and time course of T2DM less than 5 years or controls（P 0.01）.Conclusion：POT1 mRNA expression and the telomerase activity were significantly increased with the progression of T2DM;they might be the important evidences in disease prognosis.