葡萄柚漱口液对变异链球菌抗菌机制的研究

In vitro study of antibacterial activity and mechanism of grapefruit seed extract mouthwash on Streptococcus mutans

目的:研究葡萄柚漱口液对变异链球菌的抗菌活性,初步探讨其抗菌机制。方法:采用液体稀释法测定葡萄柚漱口液对变异链球菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC);同时扫描电镜下观察葡萄柚漱口液对变异链球菌细胞壁的影响。结果:葡萄柚漱口液对变异链球菌的MIC为46.875 mg/L,MBC为93.75 mg/L。扫描电镜观察显示,葡萄柚漱口液处理后的变异链球菌出现细胞壁破裂,内容物溢出。结论:葡萄柚漱口液对体外培养的变异链球菌有明显的抑制和杀灭作用,其抑制机制可能与葡萄柚提取物破坏细胞壁结构的完整性有关。

Objective：To investigate the antibacterial activity of grapefruit seed extract mouthwash against Streptococcus mutans and explore the potential mechanism.Methods：The minimal inhibitory concentration（MIC） and the minimal bactericidal concentration（MBC） of the grapefruit seed extract mouthwash against S.mutans were detected using double dilution.And the cell wall alteration of S.mutans treated with grapefruit seed extract mouthwash was observed by using a scanning electron microscope（SEM）.Results：The MIC and MBC determined for grapefruit seed extract mouthwash were 46.875 mg/L and 93.75 mg/L.Both rupture of the cell wall and release of the intracellular components were observed in the grapefruit seed extract mouthwash-treated group by SEM.Conclusion：The grapefruit seed extract mouthwash can inhibit the growth of S.mutans and kill them in vitro,this suggests that grapefruit seed extract mouthwash has an antibacterial action on the S.mutans by affecting the normal structure and function of the cell wall of S.mutans.