摘要
[目的]研究水浴提取和超声提取2种方法测定大野荞植物叶中的黄酮含量。[方法]采用水浴提取和超声提取2种方法,用紫外分光光度计测定大野荞叶中总黄酮含量。[结果]测定大野荞物叶中黄酮含量的最适波长为420nm,超声提取法和水浴提取法所得大野荞叶中平均总黄酮含量分别为5.26%和5.40%,且水浴提取法所得平均总黄酮含量极显著高于超声提取法。[结论]该研究结果对改进荞麦黄酮含量测定方法具有重要意义。
[Objective] The aim was to study the flavonoid content of Fagopyrum megaspartanium leaves by water bath and ultrasonic extractions.[Method] By using water bath extraction and ultrasonic extraction,the total flavonoid content in buckwheat leaves was measured with UV spectrophotometer.[Result] The optimum wavelength for determination of the content of flavonoid extracted from buckwheat leaves was 420 nm;the content of total flavonoid extracted by ultrasonic extraction and water bath extraction from buckwheat were 5.26% and 5.40% respectively;and the average total flavonoid content by water bath extraction was significantly higher than that by ultrasonic extraction.[Conclusion] The result is significant for the improvement of flavonoid content measurement of buckwheat.
基金
Supported by National Key Technology Research and Development Program(2006BAD02B06)
National Natural Science Foundation of China(31060207)
Agricultural Research Project of Guizhou Province[NY(2010)3094]
Special Program of Plant and Animal Breeding of Guizhou Province[No.(2010)023]~~
关键词
大野荞
超声提取法
水浴提取法
黄酮
Fagopyrum megaspartanium
Ultrasonic extraction
Water bath extraction
Flavone
