摘要
目的:建立HPLC法测定菊苣中黄酮类成分含量的方法。方法:色谱柱:Scienhome C18(4.6mm×200mm,5μm),检测波长350nm;柱温30℃;流速1mL/min。结果:木犀草素-7-O-β-D-葡萄糖苷在0.0024～0.0192μg内线性良好,y=2000000x-443.39r,=0.9999,回收率为102.7%,RSD为0.82%;木犀草素在0.01464-0.11712μg内线性良好,y=2000000x-2058.1r,=0.9999,回收率为103.4%,RSD为0.52%。结论:采用该方法进行含量测定准确可靠,重现性好。
Objective： To develop a HPLC method for the determination of Flavonoids in Cichorium intybus L.Methods：HPLC was carried out on a Purospher star Scienhome C18（4.6mm×200mm,5μm） at 30℃,at a flow rate of 1.0 mL /min.The detection wavelength was 350 nm.Results：The linearity of luteolin-7-O-β-D-glucoside was found in the range of 0.0024-0.0192μg,r=0.9999,the average recovery was 102.7%,RSD= 0.82%;The linearity of luteolin was found in the range of 0.01464-0.11712μg,r=0.9999,the average recovery was 103.4%,RSD= 0.52%.Conclusion：This method is reliable,accurate and suitable for the determination of Flavonoids in Cichorium intybus L.
出处
《农垦医学》
2010年第6期490-493,共4页
Journal of Nongken Medicine
基金
国家重大新药创制项目(2010ZX09401-304)
关键词
HPLC
菊苣
黄酮
HPLC
Cichorium intybus L.
Flavonoids
