硫化氢对慢性低氧高二氧化碳模型大鼠学习记忆的影响

Hydrogen Sulfide on Rats'Learning Memory Deficit by Induced Chronic Hypoxic Hypercapnia

目的:探讨硫化氢对慢性低O_2高CO_2模型大鼠学习记忆的影响。方法:经Morris水迷宫训练淘汰后的24只SD大鼠随机分为3组:正常对照(NC)组,低O_2高CO_2+生理盐水(HHSS)组,低O_2高CO_2+硫氢化钠(HHSH)组。HHSH组和HHSS组每天置常压低O_2高CO_2舱内8h,每周6d,共4周观察,4周后Morris水迷宫检查空间学习记忆变化,测定大鼠肺动脉平均压(mPAP)和右心室壁(RV)/左心室加室间隔(LV+S)比值、血浆中硫化氢浓度。结果:①HHSS组与NC组相比,mPAP和RV/(LV+S)比值升高,血浆中硫化氢浓度降低,平均逃避潜伏期和游泳总距离延长,穿越平台的次数减少(均P<0.05);②HHSH组与HHSS组相比,mPAP和RV/(LV+S)比值降低,血浆中硫化氢浓度升高,平均逃避潜伏期和游泳总距离缩短(均P<0.05)。结论:硫化氢可以改善慢性低O_2高CO_2模型大鼠的学习记忆障碍。

Aim：To explore the effect of hydrogen sulfide on learning memory deficit in rats induced by chronic hypoxic hypercapnia.Methods：Twenty-four male Sprague-Dawley rats selected by Morris water maze were randomly divided into three groups（8 in each group）：normal control（NC） group,hypoxic hypercapnia ＋ saline solution（HHSS） group,hypoxic hypercapnia ＋ sodium hydrosulfide（HHSH） group.The rats were received the intraperitoneal injecion with sodium hydrosulfide in the HHSH group and saline solution in the HHSS group.At the end of 4th week,all rats were tested by Morris water maze.Then the mPAP was measured by external jugular vein cannula,the ratio of the right ventricle（RV） to the left ventricle＋septum（LV＋S） was calculated,and the hydrogen sulfide concentration in the plasma was measured.Results：①Compared with the NC group,the value of mPAP and RV/（LV＋S） in the HHSS group was higher（P0.05）,the level of hydrogen sulfide in the plasma was lower（P0.05）,the escape latency to find the platform and swimming travel distance were longer,times of crossing the plantform were much reduced（P0.05）.②Compared with the HHSS group,the value of mPAP and RV/（LV＋S） in the HHSH group was lower（P0.05）,the level of hydrogen sulfide in the plasma was higher（P0.05）,the escape latency and swimming travel distance were reduced（P0.05）.Conclusioa：Rats＇ learning and memory deficit exposed to chronic hypoxic hypercapina may be ameUorated by hydrogen sulfide supplementation.