南海北部海域底泥微生物的腐殖质还原性能研究

The humic-reducing characteristics of microorganisms in sediments of northern South China Sea

微生物的腐殖质还原过程自1996年发现以来,日益成为环境领域的一个研究热点。以南海北部海域的8个底泥为实验材料,利用蒽醌-2,6,-双磺酸（AQDS）为腐殖质模式物,初步探讨了南海北部8个底泥培养物对腐殖质的还原能力,并探讨了驯化后的8个底泥微生物对腐殖质的还原过程。结果发现：从南海北部深海海域到海陆交接的香港米浦红树林的8个底泥样品培养液均能很好的还原AQDS;驯化后的8个站点底泥微生物对腐殖质还原的能力有所不同,在48 h,E425站点培养液中的OD450只有0.74,其余7个站点培养液中的OD450都在2.0~3.0之间,推测其原因是8个站点中腐殖质还原微生物的数量具有明显差异,使得各站点的OD450差异很大。研究结果为认知腐殖质还原微生物的分布和探究腐殖质还原微生物在环境中的生态学意义提供重要的理论依据。

The study of microbial humus reduction has become into a major scientific focus since it was firstly discovered in 1996.In this paper,we analyzed preliminarily the characteristics of microbial humus reduction to 8 sediment samples,which were isolated from the deep sea of northern South China Sea to the MaiPo Mangrove of Hong Kong,with anthraquinone-2,6-disulfonate（AQDS） as humus model compound.Furthermore,we investigated the humus reduction capability to the enriched sediment cultures.Experimental results showed that 8 sediment cultures could all reduce humus but have different reductive ability.Within 48 hrs,OD450 of the incubation with sediment culture from station（E425） was only 0.74,while OD450 of the incubations with other 7 sediment cultures were between 2.0 and 3.0.These results imply that there is different amount and activity of humus-reduction bacteria in different habitat.This study has some significance to understand the distribution and roles of humus-reduction bacteria in marine environment.