厚朴与远志配伍对大鼠胃肠道消化功能的影响

Magnolia officinalis and Polygala compatibility on digestive function in rats

目的:考察厚朴与远志配伍对胃肠道消化液分泌及其酶活性的影响。方法:采用幽门结扎法收集大鼠胃液,检测厚朴与远志单品以及二者配伍1:1、1:2、1:3组的胃液含量及胃酸;并采用分光光度法检测各给药组对大鼠胃蛋白酶、胰蛋白酶的影响。结果:①单味远志组能显著减少胃液分泌,降低胃液酸度,并能显著抑制胃蛋白酶活性,对胰蛋白酶活性影响不显著。②与远志组比较,单味厚朴组、远志与厚朴配伍1:2组能显著增加胃液分泌,提高胃液酸度,其中配伍1:2、1:3组有增加胰蛋白酶活性的作用趋势,但无显著性差异。③单味厚朴,厚朴与远志各配比组均能显著抑制胃蛋白酶活性。结论:单味远志能显著抑制消化功能,而当与厚朴配伍,尤其两倍于远志(即远志与厚朴1:2)时,能显著降低远志的消化功能抑制作用。提示临床使用远志,宜与2倍量厚朴配伍,以保护消化功能。

Objective： To explore the affect of gastrointestinal digestive Radix Polygalae and Magnolia.Methods ： Collecting rat gastric juice with pylorus ligation method.To detect the gastric contents and gastric acid of Polygala and Magnolia both single product and the compatibility group secretions and enzymes activities made from the compatibility between of 1：1,1：2,1：3.To detect the affect of drug-treated rats pepsin,trypsin and others by spectrophotometry.Results： ① Single Polygala group could significantly reduce the secretion of gastric juice,decreased gastric acidity,and inhibit the activity of pepsin,but trypsin activity was not significant.② Compared with the Polygala group,single Magnolia Group,the compatibility group of 1：2 between Magnolia and Polygala could significantly increase the secretion of gastric juice,increased gastric acidity.Among them,the compatibility group of 1：2,1：3 increased trypsin activity of the trend,but no significant difference.③Single Magnolia,magnolia officinalis and Polygala compatibility groups could significantly inhibit the pepsin activity.Conclusion： Single Polygala digestive function was significantly inhibited,but compatibility with Magnolia,especially twice in Polygala（Polygalaceae and Magnolia officinalis that volume ratio of 1：2）,could significantly reduce the inhibition of Polygala digestion.These results suggest that,for the protection of digestive function,the clinical use of Polygala,appropriate compatibility with the two times of Magnolia.