白癜风患者外周血白介素17和孤独核受体的表达

Expressions of interleukin 17 （IL-17） and RORγt in peripheral blood of patients with vitiligo

目的 探讨Thl7细胞在白癜风免疫发病机制中的作用.方法 采用双抗体夹心ELISA法检测白癜风患者进展期、稳定期和正常人外周血血清中白介素17（IL-17）水平;采用实时荧光定量RT-PCR方法检测外周血单一核细胞中孤独核受体（RORγt）mRNA表达水平,并分析其与疾病活动的相关性.结果 进展期与稳定期白癜风外周血中IL-17、RORγt表达水平与正常人对照组相比显著升高（P〈0.01）.结论 Th17细胞可能与白癜风的发病机制密切相关.

Objective To investigate the roles of Thl7 cells in the immunological pathogenesis of vitiligo. Methods Forty patients with progressive or stable vitiligo and 20 normal human controls were included in this study. Enzyme-linked immunosorbent assay （ELISA） was performed to measure the level of serum IL-17, and SYBR Green I real-time RT-PCR to detect the mRNA expression of RORγt in peripheral blood mononuclear cells （PBMCs） from these patients. The correlation of the above parameters with disease activity was assessed. Results The levels of serum IL-17 and ROR-yt mRNA in PBMCs were significantly higher in patients with progressive and stable vitiligo than in normal human controls （all P〈 0.01）. Conclusion Thl7 cells may be closely associated with the pathogenesis of vitiligo.