外用茶油对变应性接触性皮炎发生的影响

Effects of topical application of camellia oil on the development of allergic contact dermatitis

目的 观察茶油对小鼠变应性接触性皮炎发生的影响.方法 建立二硝基氟苯致小鼠变应性接触性皮炎模型,外用药物进行治疗,观察发生皮炎的小鼠耳片的组织学变化（HE染色）,RT-PCR检测组织中炎症细胞因子IL-2、IFN-γmRNA的表达水平.结果 成功建立二硝基氟苯致小鼠变应性接触性皮炎的动物模型,且皮肤中IL-2、IFN-γmRNA的表达显著增高.哈西奈德溶液组和茶油组的耳片组织肿胀明显减轻,炎症反应轻微,炎性细胞浸润数目少,组织病理变化轻.在茶油和哈西奈德溶液的干预下,炎性组织IL-2、IFN-γ的mRNA表达均不同程度下降.结论 茶油减轻二硝基氟苯致小鼠变应性接触性皮炎的病理损害,下调了炎症因子的表达.

Objective To observe the effects of camellia oil on the development of allergic contact dermatitis in mice. Methods Mice models of allergic contact dermatitis were established with dinitrofluo-robene （DNFB）. Then, certain concentrations of camellia oil and halcinonide were used topically on the ears of mouse models twice daily for 6 days. Skin specimens were obtained from the treated ears and subjected to histology examination and pathological analysis. RT-PCR was performed to detect the mRNA expressions of interleukin 2 （IL-2） and interferon γ （IFN-γ） in the skin tissues. Results Allergic contact dermatitis was successfully induced by DNFB in mice with a marked increase in the mRNA expression of IL-2 and IFN-7. There was a slight decrease in the ear swelling degree, inflammatory reaction intensity, number of infiltrating inflammatory cells, and pathological severity in camellia oil-treated mice and halcinonide-treated mice compared with glycerinetreated mice. Camellia oil and halcinonide also induced different degrees of reduction in the mRNA expression of IL-2 and IFN-γ in inflammatory tissues. Conclusion Camellia oil can alleviate the pathological damage and down-regulate the expression of IL-2 and IFN-7 in mice with DNFB-induced allergic contact dermatitis.