不同基本培养基对南方高丛蓝浆果丛生枝增殖及生长的影响

Effect of different basic media on proliferation and growth of clumpy shoot of southern highbush blueberry (Vaccinium corymbosum hybrids)

比较了MWPM和WPM单一培养基以及MS-WPM、MS-MWPM、MWPM-WPM等体积混合培养基(均含2 mg·L-1 ZT、20 g·L-1蔗糖和7 g·L-1琼脂,pH 5.0)对南方高丛蓝浆果(Vaccinium corymbosum hybrids)品种‘南月’(‘Southmoon’)优选系A47和A167丛生枝增殖及生长的影响。实验结果显示:在MS-WPM和MS-MWPM混合培养基上,优选系A47和A167丛生枝的增殖倍数、总长度及叶片叶绿素含量均高于或显著高于MWPM和WPM单一培养基,丛生枝的鲜质量、干质量和含水量与MWPM和WPM单一培养基相当;而在MWPM-WPM混合培养基上,优选系A47和A167丛生枝的增殖倍数在5种培养基中虽然不是最低的,但丛生枝的各项生长指标均较低或最低。综合分析结果表明:MS-WPM和MS-MWPM混合培养基较目前常用的WPM和MWPM单一培养基更适宜于南方高丛蓝浆果品种‘南月’优选系A47和A167丛生枝的增殖培养。

Effects of single media MWPM and WPM,and mixed media MS-WPM,MS-MWPM and MWPM-WPM with equal volume（all containing 2 mg·L-1 ZT,20 g·L-1 sucrose and 7 g·L-1 agar,pH 5.0） on proliferation and growth of clumpy shoot of superior strains A47 and A167 of southern highbush blueberry（Vaccinium corymbosum hybrids） cultivar ‘Southmoon’ were compared.The results show that on mixed media MS-WPM and MS-MWPM,proliferation times,total length and leaf chlorophyll content of clumpy shoot of superior strains A47 and A167 all are higher or significantly higher than those on single media MWPM and WPM,fresh and dry weights and water content of clumpy shoot are equivalent to those on single media MWPM and WPM.While on MWPM-WPM mixed medium,although proliferation times of clumpy shoot of superior strains A47 and A167 are not the lowest among five media,but all growth indexes of clumpy shoot are lower or the lowest.Comprehensive analysis results indicate that mixed media MS-WPM and MS-MWPM are better than common single media WPM and MWPM for proliferation of clumpy shoot of superior strains A47 and A167 of southern highbush blueberry cultivar ‘Southmoon’.