蛛网膜下腔置管灌注rhNCAM1促损伤脊髓再生修复的实验研究

Effects of administration of rhNCAM1 protein into the subarachnoid space on the regeneration following spinal cord injury

目的：探讨神经细胞粘附分子1（neural cell adhesion molecule 1,NCAM1）灌注治疗小鼠脊髓撞击伤后对脊髓修复及神经功能恢复的影响。方法：采用改良NYU（纽约大学）Weight-Drop Impactor打击装置,以25 gcf（l0 g重量从25 mm高度自由落下）制备脊髓损伤模型（打击面积为2 mm×2 mm）。将SA小鼠30只随机分为3组：实验组（rhNCAM1组,n=10）于损伤即刻经蛛网膜下腔注入rhNCAM1蛋白2μl（约100μg）,隔日1次,共5次;对照组（生理盐水组,n=10）于损伤即刻经蛛网膜下腔注入生理盐水2μl,隔日1次,共5次;假手术组仅做椎板切除术,术后缝合切口。观察各组小鼠18周行为学改变,行BBB评分。第8周灌注固定、取材,采用神经纤维200（NF200）免疫荧光检测,进行图像分析及统计处理。结果：（1）行为学评估：实验组BBB评分在各时间点均显著高于对照组（P〈0.01）;（2）NF200免疫荧光染色：实验组阳性着色明显多于、并强于对照组;实验组无组织瘢痕结构面积明显小于对照组。结论：蛛网膜下腔应用rhNCAM1后可能改善SCI后损伤区及两端的神经细胞之间的功能,促进轴突再生,促进双下肢运动功能的恢复。

Objective：To investigate the effects of rhNCAM1 on recovery of spinal cord injury（SCI）.Methods：The rhNCAM1 protein was immediately administrated into the subarachnoid space of mice after creating a SCI by an improved version of NYU（New York University） Weight-Drop Impactor.Thirty adult SA mouse were randomly divided into 3 groups,n=10 for each group.rhNCAM1 group：spinal cord injury combined with treatment of rhNCAM1 by perfusion;control group：spinal cord injury combined with treatment of normal saline by perfusion;sham operation group：the animal only subjected to laminectomy.Immunofluorescence staining of NF200 and behavioral manifestation was observed to analyze the effects of rhNCAM1 on recovery of SCI.Results：The hindlimb motor recovery of rhNCAM1 group was better than that in the normal saline group.Neurofilament（NF200） immunofluorescence staining showed that the positive staining in rhNCAM1 group was significantly more than that in the control group.Conclusion：The results indicate that the NCAM protein may play a role in the recovery of SCI.