摘要
目的:探讨三氧化二砷(As2O3)对人卵巢癌细胞系SKOV3细胞侵袭转移能力及其对尿激酶型纤溶酶原激活物(uPA)及尿激酶受体(uPAR)表达的影响。方法:采用0.5μmol/L、1μmol/L、2μmol/L 3种浓度的As2O3处理人卵巢癌SKOV3细胞,48h后收集细胞,采用Transwell检测细胞的侵袭转移能力,实时定量PCR及免疫细胞化学方法检测uPA、uPAR mRNA及蛋白表达的变化。结果:经不同浓度As2O3处理的细胞,穿过模拟基底膜的数目逐步减少,As2O3明显抑制SKOV3细胞的侵袭转移能力(P<0.05);细胞uPA及uPARmRNA及蛋白表达水平与对照组相比显著降低(P<0.05),其表达水平随着药物浓度的增加而降低。结论:As2O3可抑制卵巢癌细胞的侵袭转移能力,其机制可能与抑制uPA、uPAR的表达有关。
Objective：To explore the effects of aresenic trioxide（As2O3） on the invasion and metastasis to SKOV3 cells and the expression of urokinase type plasminogen activator（uPA） and it＇s receptor（uPAR）.Methods：SKOV3 cells were treated with 0.5μmol/L,1μmol/L and 2μmol/L As2O3 for 48 hours respectively.The suppressing effect on the invasion and metastasis to SKOV3 cells was detected by Transwell invasion chamber.The expression level of uPA and uPAR mRNA and protein were detected by real-time quantitative PCR and SABC immune-tissue-chemistry method.Results：The number of cells which through the simulative basement membrane is decreased after treated by As2O3.The invasion and metastasis ability of passing the transwell invasion chamber decreased in SKOV3 cells which treated by As2O3（P0.05）,and the expression level of uPA,uPARmRNA and protion of SKOV3 cells were lower in comparison with untreated cells in a dosage-dependent manner（P0.05）.Conclusion：SKOV3 cells,invasion and metastasis ability can be inhibited by As2O3.The mechanism might be associated with the reduction of uPA and uPAR expression.
出处
《现代妇产科进展》
CSCD
北大核心
2011年第3期225-228,共4页
Progress in Obstetrics and Gynecology
