^(125)I联合ADM对乳腺癌MCF-7细胞增殖、凋亡的影响

Effects of radioactive seeds ^(125)I combined with adriamycin on proliferation and apoptosis of breast cancer MCF-7 cells in vitro

目的研究放射性粒子125I联合化疗药物多柔比星(阿霉素,ADM)对乳腺癌MCF-7细胞增殖、凋亡的影响。方法按2×2析因设计将乳腺癌敏感株MCF-7细胞随机分成A、B、C、D 4组,A组:空白对照组;B组:单纯125I粒子组;C组:单纯ADM组;D组:125I粒子+ADM组。采用流式细胞术检测各组干预后的细胞周期分布及细胞凋亡率。结果 (1)单纯125I粒子近距离低剂量率持续照射后将MCF-7细胞阻滞于G2~M期1。25I联合ADM将MCF-7细胞周期主要集中在G0~G1期,同时伴有较大比例的细胞凋亡。(2)各组细胞的早期凋亡率分别为:A组(0.99±0.05)%、B组(19.22±4.92)%、C组(16.57±4.73)%、D组(3.16±1.08)%;各组晚期凋亡及坏死率为:A组(0.32±0.18)%、B组(3.16±1.39)%、C组(3.24±0.75)%、D组(28.99±7.96)%,D组晚期凋亡及死亡率明显提高。结论 125I放射性粒子能有效诱导乳腺癌细胞凋亡,与化疗药物ADM联合作用除诱导细胞凋亡,还可导致大量细胞死亡,具有协同、增效的作用。

Objective To investigate the effects of 125I radioactive seeds combined with adriamycin（ADM） on proliferation and apoptosis of breast cancer MCF-7 cells in vitro.Methods The cultured breast cancer MCF-7 cells were randomly divided into four groups by 2×2 factorial design： 125I group,ADM group,125I combined with ADM group and control group.Cell cycle distribution and apoptosis of MCF-7 cells were analyzed by flow cytometry（FCM）.Results MCF-7 cells were arrested at G2~M phase in 125I group,and arrested at G0~G1 phase with larger proportion of apoptotic cells in 125I combined with ADM group.Flow cytometry and Annexin V staining showed that the earlier period apoptosis rates of MCF-7 cells were（19.22±4.92）% in 125I group,（16.57±4.73）% in ADM group.In 125I with ADM group,the earlier stage apoptosis rate declined to（3.16±1.08）%,however,the rates of advanced stage apoptosis and cells necrosis were raised to（28.99±7.96）%.Conclusions The radioactive seeds125I induces apoptosis of MCF-7 cells.125I combined with ADM causes the cell apoptosis and necrosis,showing synergestic effects on MCF-7 cells.