小鼠动物实验方法系列专题(四) Micro-CT辅助结肠造影在小鼠炎性肠病中的应用

Monitoring Colitis Development in Mice by Micro-CT Colonography

用DSS喂饲小鼠建立小鼠IBD模型,在建模的第0、8、13天分别用碘帕醇灌胃加腹腔注射的方法造影并micro-CT扫描降结肠壁厚度,结果第8天和第13天分别为O.4586±0.04 mm和0.40325±0.03 mm,显著(P<O.001)大于对照组(0.28±0.02 mm)。同时检测小鼠体重变化、临床评分、结肠病理切片并评分,发现结肠壁厚度变化趋势和常规指标一致。应用micro-CT辅助小鼠结肠造影方法,可以直观、动态、无创地对小鼠肠道进行影像学分析。

To evaluate colon wall thickness noninvasively in the dextran sodium sulfate （DSS） induced mouse model of colitis, we performed micro-CT colonography. Mice were scanned by micro-CT at day 0, day 8 and day 13 after induction of colitis and calculated the colon wall thickness. Iopamidol was used by oral and intraperitoneal injection to show the colon wall. The value of the descending colon wall thickness were 0.4586±0.04 mm （day 8） and 0.40325±0.03 mm （day 13） significantly （P〈0.001） higher than the control group （0.28±0.02 mm）. The related body weight loss, clinical score, histological section and score were consistent with the result ofmicro-CT. Thus, the results suggested that micro-CT can be used directly, consecutively and noninvasively in monitoring of the inflammatory response in mouse colitis in future studies.