原生动物伪红色双轴虫细胞纤毛器微管胞器的直接荧光标记

FLUTAX Labeling of the Ciliature Microtubular Organelles in Diaxonella pseudorubra

应用荧光紫杉醇直接荧光标记法显示,原生动物纤毛虫伪红色双轴虫(Diaxonellapseudorubra)细胞纤毛器微管中,口围带基部含小膜托架及与托架相联系的肋壁微管;额腹横棘毛基部含前纵微管束、后纵微管束、横微管束和周围微管束,其微管在不同棘毛基部的定向和发达程度不一;缘棘毛基部含前纵微管束、后纵微管束。细胞形态发生过程中,前仔虫口纤毛器微管独立发生于老口围带内侧,在细胞形态发生末期新纤毛器微管形成时,尚有部分老额棘毛、横棘毛和缘棘毛残存,此后老结构逐渐被吸收。结果表明,伪红色双轴虫的纤毛器基部微管的分化很可能具有种属级的特异性,新纤毛器微管分化过程中老结构可能具有定位和物质贡献作用。

The microtubular organelles of a hypotrichous ciliate, Diaxonella pseudorubra, were visualized by FLUTAX （fluorescence taxoid） labeling. The results showed that these organelles consisted of adoral zone of membranelles （AZM）, frontal-ventral-transverse cirri （FVTC） and the base associated microtubules of these ciliatures. The microtubular cytoskeleton of AZM comprises membranelle brackets and the associated microtubules. There are four types of microtubules at the base of FVTC, anterior longitudinal microtubules （ALM）, posterior lon- gitudinal microtubules （PLM）, transverse microtubules （TM） and radiating microtubules（RM）. These different types of microtubules differ from each other in developmental degree. The base associated microtubules of marginal cirri （MC） contain ALM and PLM. All of the old AZM were renewed during the process of morphogenesis. AZM of the proter was formed at the site where the inner of the old AZM. At the end of the differentiation of the cell, some part of old FC, TC and MC remain unchanged for a long time. Meanwhile, our results also indicated that the microtubular organization of Diaxonella pseudorubra was quite different from other ciliates. Moreover, during the period of defferetiation and maturation of the new structures, the collapse of old cirri may contribute to the new structure＇s orienting and constituting while the function of old cirri is losing gradually.