诱导多功能干细胞的影响因素

Impacting Factors for Induced Pluripotent Stem Cells

将体细胞诱导为多功能干细胞为人类的再生医学提供了一个全新的研究手段,从而可以不用损坏胚胎就能获得可用于治疗各种特殊疾病的细胞。本文比较了近年来关于生成诱导性多能干细胞(induced pluripotent stem cells,iPS细胞)的诱导方法及重编程效率,总结了这些方法的共同点;另外通过对每个不同试验过程的影响因素进行比较,归纳了影响iPS细胞重编程过程的几个因素。

Direct reprogramming of somatic cells to induced pluripotent stem cells （iPSCs） provides an invaluable resource for regenerative medicine, enabling the generation of patient-specific ceils of any lineage without the use of embryonic material. We compared the currently reported protocols, identified the essential steps common to these methods, and summarized several factors that affect iPS cells reprogramming process, with an emphasis on standardization of certain parameters for accurate comparison between independent experiments.