量子点荧光探针在人舌鳞状细胞癌组织中的应用研究

Application of quantum dots fluorescent probes in tissue of human tongue squamous cell carcinoma

目的通过免疫荧光技术,利用量子点荧光探针在人的舌鳞状细胞癌组织中检测特定蛋白,探讨量子点荧光探针在人舌鳞状细胞癌组织中的应用。方法相同粒径的量子点通过间接免疫荧光技术分别对人舌鳞状细胞癌组织中的P53及Bcl-2蛋白进行特异荧光标记,荧光显微镜观察蛋白定位表达;不同粒径的量子点通过间接免疫荧光技术,在同一张舌鳞状细胞癌组织切片上分别标记P53蛋白与Bcl-2蛋白,并对其定位表达进行观察。结果相同粒径的量子点荧光探针可分别与组织中的P53、Bcl-2蛋白结合,在紫外荧光激发下发出特异红色荧光,P53蛋白结合物主要分布于细胞核;Bcl-2蛋白结合物主要分布于细胞质;不同粒径的量子点荧光探针在同一张组织切片上可同时对P53蛋白与Bcl-2蛋白进行标记,在紫外荧光激发下2种蛋白结合物发出2种不同颜色的荧光形成鲜明对比,显示量子点荧光探针标记的蛋白定位准确,特异性高。结论量子点荧光探针可应用于人舌鳞状细胞癌组织中的2种蛋白的特异免疫荧光标记。

Objective To study if quantum dots fluorescent probes can be applied to detect P53 protein and Bcl-2 protein in tissue of human tongue squamous cell carcinoma.Methods By indirect immunofluorescence assay the same particle size quantum dots fluorescent probes were applied to detect P53 protein and Bcl-2 protein respectively.Different particle size quantum dots fluorescent probes were applied to detect P53 protein and Bcl-2 protein simultaneously in paraffin-embedded tissue section of human tongue squamous cell carcinoma under fluorescent microscope.Results P53 protein and Bcl-2 protein can be combined with quantum dots fluorescent probes and specific fluorescene can be observed with ultraviolet light excited.P53 protein was mainly distributed in the nucleus,and Bcl-2 protein major in the cytoplasm.P53 protein and Bcl-2 protein can be combined with different particle size quantum dots fluorescent probes respectively in the same paraffin-embedded tissue section of human tongue squamous cell carci-noma and two kinds of fluorescene can be observed.Conclusion Quantum dots fluorescent probes can be applied to detect two kinds of specific protein in paraffin-embedded tissue section of human tongue squamous cell carcinoma.