口腔扁平苔藓病损区白细胞介素12p40和干扰素-γ的表达及意义

Expression of interleukin-12p40 and interferon-γ in local lesions of human oral lichen planus

目的探讨白细胞介素12p40(IL-12p40)及干扰素-γ(IFN-γ)在口腔扁平苔藓(OLP)病损形成及发展中的意义。方法正常口腔黏膜组织11例,OLP组织43例,采用免疫组织化学Envision二步法检测IL-12p40和IFN-γ的表达情况,分析其与OLP患者临床病理特征的关系。结果 1)OLP组IL-12p40与IFN-γ的阳性表达率均高于正常对照组,差异有统计学意义(P<0.05)。2)在OLP组中,IL-12p40阳性表达率在IFN-γ阳性组高于IFN-γ阴性组,差异有统计学意义(掊2=5.828,P=0.016),IFN-γ和IL-12p40表达之间存在正相关关系(r=0.357,P=0.019)。3)IL-12p40的阳性表达率在病程短于6个月组高于病程长于6个月组(掊2=7.935,P=0.005);基底细胞液化程度高分组中的IFN-γ阳性表达率高于低分组(掊2=9.070,P=0.011)。结论 IL-12可能通过促进典型Th1型细胞因子IFN-γ的产生参与了OLP病损区的病理损伤过程,在OLP局部病损形成初始阶段起到关键作用。

Objective To investigate the significance of cytokine interleukin-12p40（IL-12p40） and interferon-γ（IFN-γ） in tissues formation and development of human oral lichen planus（OLP）.Methods The tissues of 11 cases of normal oral epithelium and 43 cases of OLP were investigated for the expression of IL-12p40 and IFN-γ proteins by using Envision two-step immunohistochemistry.The correlations between the expressions of these two cytokines,and their clinical and pathological significance in OLP were analyzed.Results 1）IL-12p40 and IFN-γ proteins were up-regulated in OLP comparing with that in normal oral mucosa and there was statistical significance between their difference（P0.05）.2）The percentage of positive IL-12p40 staining in OLP of IFN-γ positive group was higher than IFN-γ negative group and there was statistical significance between their difference（χ2=5.828,P=0.016）.A positive correlation was found between IL-12p40 and IFN-γ proteins in OLP（Spearman r=0.357,P=0.019）.3）The percentage of positive IL-12p40 staining in OLP with short course（6 months） was higher than that in OLP with long course（≥6 months;χ2=7.935,P=0.005）,and a significant association was found between IFN-γ over expression and the degeneration of base cells in OLP lesions（χ2=9.070,P=0.011）.Conclusion These results indicate that at the primary phase of OLP,IL-12 may drive the pathological destruction in OLP lesions by elevating IFN-γ protein locally.IFN-γ may play an important role for the pathological destruction in OLP lesions.