中药白芍总苷预防非肥胖型糖尿病小鼠自发性涎腺炎的研究

Study on total glucosides of peony preventing non-obese diabetic mice from sialoadenitis

目的探讨中药白芍总苷对非肥胖型糖尿病(NOD)小鼠自发性涎腺炎的预防作用及机制。方法 4周龄雌性NOD小鼠27只,随机分成3组:生理盐水组、羟氯喹组及白芍总苷组。自5周龄开始,将等效剂量药物溶于0.4 mL生理盐水中每天灌胃给药,直到20周龄处死。10、15及20周龄时收集每组小鼠的唾液流量以及血清、颌下腺组织。采用苏木精-伊红染色观察颌下腺组织病理学改变;酶联免疫吸附试验(ELISA)检测血清自身抗体(SSA、SSB及α-fodrin)及相关细胞因子水平。结果与生理盐水组相比,白芍总苷组及羟氯喹组小鼠治疗后唾液流量明显增加,颌下腺炎性浸润减轻,血清自身抗体水平明显下降,Th1/Th2型细胞因子表达失调有所改善。白芍总苷组和羟氯喹组间差异无显著性(P>0.05)。结论白芍总苷对NOD小鼠自发性涎腺炎有一定预防作用,其作用机制可能与药物减轻颌下腺淋巴细胞灶性浸润程度及改善Th1/Th2型细胞因子表达失调等有一定关系。

Objective To investigate the immunosuppressive effect of total glucosides of peony（TGP） on sialoadnitis in non-obese diabetic mice（NOD mice） and explore its possible mechanism.Methods 27 female five-weekold NOD mice were randomly divided into three groups： TGP,hydroxychloroquine（HCQ） and normal saline（NS） group. One week later,they were administered intragastrically in TGP,HCQ and NS respectively.Three mice from each group were sacrificed at the age of 10,15 and 20 weeks.The saliva flow,serum and submandibular glands were collected at these time points.Histological changes of submandibular glands were examined by HE staining.The expression of autoantibodies（SSA,SSB and anti-α-fodrin） and associated cytokines in serum were detected by enzyme-linked immunosorbent assay（ELISA）.Results Compared with the NS group,salivary flow was significantly increased,the extent of the histological changes were ameliorated,the autoantibodies in serum were significantly decreased and the imbalance of Th1/Th2 cytokines was remedied in the mice treated with TGP and HCQ.There were no significant differences between the two groups treated with TGP and HCQ（P0.05）.Conclusion TGP can effectively ameliorate sialoadenitis on NOD mice.The mechanism was thought to be associated with the protection of submandibular gland from intense inflammation and the correction of Th1/Th2 cytokines imbalance.