小剂量丙泊酚对食管鳞癌细胞Eca109生物学行为的影响

Effect of low dose propofol on biological behavior of esophageal squamous cell carcinoma cell line Eca109

目的:探讨低剂量丙泊酚对食管鳞癌细胞Eca109的增殖、凋亡、迁移及侵袭等生物学行为的影响。方法:根据预实验结果设置低剂量丙泊酚组,干预食管鳞癌细胞Eca109后,利用MTT、流式细胞术、transwell检测对增殖、凋亡、迁移及侵袭的影响,应用实时荧光定量PCR检测血红素氧合酶1(HO-1)的表达变化。结果:低剂量Eca109可以促进Eca109的增殖、迁移与侵袭并抑制凋亡,实时荧光定量PCR显示干预后HO-1的表达增加且随浓度的增加而增加。结论:低剂量丙泊酚可以促进Eca109的增殖、迁移与侵袭并抑制凋亡,可能与促进HO-1的表达相关。

AIM：To detect the effect of low dose propofol on the proliferation,apoptosis,migration,and invasion of esophageal squamous cell carcinoma cell line Eca109.METHODS： The proliferation,apoptosis,migration,and invasion of esophageal squamous cell carcinoma cell line Eca109 were detected by MTT assay,flow cytometry,transwell assay respectively.The effect of low dose propofol on expression of heme oxygenase-1（HO-1） was confirmed by Real-time quantitative PCR.RESULTS： Low dose propofol could inhibit the proliferation,migration,invasion and promate the apoptosis of esophageal squamous cell carcinoma cell line Eca109.And low dose propofol increased the expression of HO-1 mRNA in a dose-dependment manner.CONCLUSION： Low dose propofol affects the biological behavior of esophageal squamous cell carcinoma cell line Eca109,which has a relationship with increasing the expression of HO-1.