白细胞介素-6基因-572C/G多态性与慢性牙周炎的相关性研究

Association Between IL-6-572C/G Polymorphism and Chronic Periodontitis

目的研究白细胞介素-6(interleuk in-6,IL-6)基因启动子区域-572C/G位点基因多态性与慢性牙周炎易感性的关系。方法采用病例对照试验设计,轻中度牙周炎组87例,重度牙周炎组72例,健康对照组90例,收集颊粘膜拭子,使用聚合酶链反应—限制性内切酶片段长度多态性基因分析的方法,检测各组IL-6-572位点基因型和等位基因分布。结果 IL-6基因-572C/G位点G等位基因的检出率在健康组是14.4%,轻中度牙周炎组是14.4%,重度牙周炎组是20.1%。等位基因频率在患者和健康者之间无统计学差异(P=0.287)。CC基因型在各组的分布分别是健康组73.3%,轻中度牙周炎组71.3%,重度牙周炎组63.9%,各组之间差异无统计学意义(P=0.308)。结论 IL-6基因-572位点多态性与汉族人群慢性牙周炎的易感性无明显相关。

Objective The aim of this study was to investigate whether or not the IL-6-572C/G polymorphism influence the occurrence and development of chronic periodontitis.Methods The genotype of IL-6 in a case-control study including 87 patients with mild chronic periodontitis,72 patients with advanced chronic periodontitis and 90 healthy controls were analyzed by polymerase chain reaction-restriction fragment length polymorphism.Distribution of alleles and genotypes were calculated by the chi-square test.Results the G-allele frequencies at the-572 promoter region of IL-6 were 14.4% in group with no or mild chronic periodontitis and 20.1% in group with advanced chronic peridontitis.Comparison of genotypes and alleles revealed no significant difference between patients and controls.Conclusion It suggested that there was no relationship between chronic periodontits and IL-6-572 polymorphism in Chinese Han population.