人骨髓基质干细胞对人牙周膜干细胞膜片再生牙周膜样组织的影响

The influence of human bone marrow mesenchymal stem cells on human periodontal ligament stem cell sheet in regenerating periodontal-like tissue

目的:研究人骨髓基质干细胞(human bone marrow mesenchymal stem cells,HBMMSCs)对人牙周膜干细胞(human periodontal ligament stem cells,HPDLSCs)膜片再生牙周膜样组织的影响。方法:利用Transwell共培养HBMMSCs和HPDLSCs,通过ALP和MTT的方法检测共培养HPDLSCs的ALP活性和增殖活性。将共培养的HPDLSCs制成膜片与煅烧的陶瓷牛骨(ce-ramic bovine bone,CBB)复合,行裸鼠体内异位移植。结果:体外结果显示,HBMMSCs可以提高HPDLSCs的ALP活性,促进细胞增殖。体内结果显示,共培养的HPDLSCs膜片不仅可以再生含矿化结构的牙周膜样组织,而且再生出丰富的血管。结论:HBMMSCs促进HPDLSCs膜片再生血管化的牙周膜样组织。

Objective： To study the influence of human bone marrow mesenchymal stem cells（HBMMSCs） on human periodontal li-gament stem cell（HPDLSC） sheet in regenerating periodontal-like tissue.Methods： HBMMSCs and HPDLSCs were co-cultured in transwell.The co-cultured HPDLSCs（experimental group） were examined by alkaline phosphatase（ALP） and MTT assays.The con-ventionaly cultured HPDLSCs were used us the control.Then,the co-cultured HPDLSCs were cultured to form stem cell sheets which were combined with calcinated ceramic bovine bone（CBB） for immunodeficient mice transplantation.Results： In vitro HBMMSCs could promote ALP activity and cell proliferation.In vivo,co-cultured HPDLSCs can not only obtain mineralized periodontal-like tissue,but also regenerated affluent blood vessels.Conclusion： HBMMSCs may promote vascularized periodontal-like tissue regeneration by HPDLSCs sheet.