过氧化苯甲酰对小鼠肾脏损伤的实验研究

Experimental Study on Benzoyl Peroxide Induced Renal Damage in Mice

目的研究面粉增白剂过氧化苯甲酰(benzoyl peroxide,BPO)对小鼠肾脏的毒性。方法将64只清洁级昆明小鼠随机分成4组,分别为对照(玉米油)组和低(50 mg/kg)、中(100 mg/kg)、高(200 mg/kg)剂量BPO染毒组,每组16只,雌雄各半。采用经口灌胃的方式进行染毒,染毒容量为5 ml/kg,每天1次,连续染毒6周。染毒结束后,测定肾组织匀浆上清液中的超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量,定量观察坏死细胞与梗死灶数目,并计算坏死细胞率。结果与对照组相比,仅高剂量BPO染毒组小鼠SOD活力降低,MDA含量增高,差异均有统计学意义(P<0.05)。低、中、高剂量BPO染毒组小鼠肾脏细胞坏死率均高于对照组,差异均有统计学意义(P<0.05)。高剂量BPO染毒组坏死细胞率高于低、中剂量BPO染毒组,差异均有统计学意义(P<0.05)。高剂量组16只小鼠病理切片共观察到13处梗死灶。结论在一定剂量范围内,BPO可以损伤小鼠体内的抗氧化系统,导致肾脏出现不同程度的氧化损伤。

Objective To explore the renal damage induced by benzoyl peroxide （BPO） in mice. Methods Sixty-four healthy Kunming mice were randomly divided into four groups： control group,low-dose group,moderate-dose group and high--dose group. The mice in the control group were given corn oil only,while BPO （5 ml/kg） was given to the other grqups by i.g. once a day for six consecutive weeks. The three groups were treated with low,medium and high dosages of 50, 100 and 200 mg/（kg.d） through garage. The kidneys were collected after six weeks treatment. Superoxide dismutase （SOD） activity and malondialdehyde （MDA） content were measured in one ,and the other one was used to do pathological examination. Results Compared with the control group,SOD activities was lower in high-dose groups ,while MDA contents was higher than those in the low, moderate and control groups. Renal injury could be observed in the pathological section by degrees. Necrosis rates of each group were higher than the control group and high-dose group was higher than those in the low,middle groups. Thirteen infarctions were observed in the high dose group. Conclusion In a certain range ,BPO may damage the antioxidant system,resulting in different degrees of renal oxidative damage in mice.