金雀异黄素对SGC-7901胃癌细胞超微结构及极光激酶A基因表达的影响

Effect of Genistein on Nano-Microstructure and Expression of Genes Aurora-A in Cultured SGC-7901 Gastric Carcinoma Cells

应用原子力显微镜(Atomic Force Microscope,AFM)技术、Real-time PCR及Western blotting方法,分别检测金雀异黄素(genistein)对SGC-7901胃癌细胞超微结构、Aurora-A基因mRNA和蛋白表达的影响。AFM扫描显示,经20μg/mLgenistein处理SGC-7901胃癌细胞48 h,细胞的超微结构呈现凋亡形态特征改变;Aurora-A表达分析,5μg/mL组、10μg/mL组和20μg/mL组与对照组比较,其mRNA表达量分别下降(31.6±0.02)%、(38.8±0.04)%和(59.9±0.02)%,其中5μg/mL组与10μg/mL组间比较无显著性差异(P>0.05),其余组间比较均有显著性差异(P<0.05);其蛋白表达量分别下降(26.8±0.04)%、(33.0±0.10)%和(48.5±0.09)%,组间比较均有显著性差异(P<0.05)。由此推断金雀异黄素诱导SGC-7901胃癌细胞凋亡的分子机制可能涉及极光激酶A mRNA及蛋白表达下调。

The nano-microstructure of SGC-7901 gastric carcinoma cells was determined by Atomic Force Microscope;the expression of Aurora-A mRNA and protein were evaluated by Real-time PCR and Western blotting respectively.The nano-microstructure of SGC-7901 gastric carcinoma cells altered apparently,showed apoptosis during the treatment of 20 μg/mL genistein after 48 h.Compared with group of control,Aurora-A mRNAs were down-regulated after genistein treatment,which were（31.6±0.02）%,（38.8±0.04）%,（59.9±0.02）%,between the group of 5 μg/mL and the group of control,there was no significant difference（P0.05）,while among other groups,there were significant differences（P0.05）;Aurora-A proteins were also down-regulated,which were（26.8±0.04）%,（33.0±0.10）%,（48.5±0.09）%,there were significant differences among all the group（P0.05）.The present study indicated that the mechanism of SGC-7901 gastric carcinoma cells apoptosis induced by genistein may be due to the down-regulation of expression of Aurora-A mRNA and protein.