GC-LRMS法测定啤酒中3种N-亚硝胺化合物

Determination of three nitrosamines in beer by GC-LRMS

用气相色谱-低分辨率质谱（GC-LRMS）联用法测定了啤酒中的3种亚硝胺。通过与脱亚硝基化试剂（HBr＋HAc）作用，脱去亚硝基生成相应的二级胺，再与对甲苯磺酰氯反应，然后进行GC-LRMS分析。与原N-亚硝胺化合物相比，有效地改善了N-亚硝胺的色谱行为，衍生化后目标化合物的检测限降低了1～2个数量级（低至0.05μg/L）。应用本方法成功地测定了啤酒中的3种亚硝胺化合物。

A gas chromatography with low-resolution mass spectrometry （GC-LRMS） has been used for the determination of three nitrosamines in beer. In order to improve their chromatographic behavior by denitrosation reagents （HBr ＋ HAc ） to produce corresponding secondary amines and followed with reaction with p- Toluenesulfonyl chloride, the final derivatives were analysed by GC-LRMS, Compared with original nitrosamines, it showed that limits of detection had been decreased in 1 or 2 order of magnitude, at the level of 0. 05 μg/L by derivatization. The method were successfully applied to detection of 3 nitrosamines in beer