DBP在难治性癫痫患者脑组织中的异常表达

Abnormal Expression of Albumin D-site-binding Protein in Brain of Patients with Intractable Epilepsy

目的通过观察白蛋白D-位点结合蛋白(DBP)和丝裂原活化蛋白激酶(MAPK)在难治性癫痫(IE)患者脑组织中的表达,探讨其在癫痫的发生、发展中的作用。方法用免疫组织化学及DBP/MAPK荧光双标法检测35例难治性癫痫患者手术后脑组织标本中DBP、MAPK的表达,并与15例对照组进行比较。结果 DBP主要在神经元表达,MAPK在神经元和胶质细胞中均有表达,主要分布于神经元,且IE中表达(DBP 0.4737±0.0262、MAPK 0.2935±0.0106)高于对照组(DBP 0.2476±0.0229、MAPK0.2370±0.0125,P<0.05),荧光双标显示DBP与MAPK分布在同一神经元上。结论 DBP和MAPK在难治性癫痫患者手术后脑组织标本中表达上调,且共同表达在同一细胞上,提示两者在难治性癫痫的发生发展中可能存在一定的相互作用。

Objective The mechanisms that under/ie the pathogenesis of intractable epilepsy （IE） are still unclear. The expression of albumin D-site-binding protein （DBP） and mitogen-aetivated protein kinases （MAPK） in the temporal lobes of patients with IE are investigated,in order to explore the possible roles of DBP in the pathogenesis of IE. Methods Expressions of DBP and MAPK were detected by immunohistochemistry and double-label imrnunofluoreseenee staining with DBP/MAPK in 35 patients with IE. The data were compared with those of 15 eontrols. Results DBP expression in IE （0. 473 7 ± 0. 026 2 ） was significantly higher compared with eontrols（ 0. 247 6 ±0. 022 9, P 〈 0. 05 ）. MAPK expression in IE （0. 293 5 ± 0. 010 6 ） was likewise higher compared with controls （0. 237 0 ± 0. 012 5, P 〈 0. 05 ）. DBP and MAPK were mainly expressed in the cytoplasm of neurons. The double-label immunofluoreseenee staining showed that DBP and MAPK occurred in the same neurons. Conclusions The expression of DBP and MAPK in epilepsy patients was up-regulated. These may suggest a possible pathogenetie role in IE.