水浴振荡辅助酶法提取双孢蘑菇多糖的工艺研究

Study on extraction of polysaccharides from Agaricus bisporus by shaking bath-assisted enzymatic mothod

为了提高双孢蘑菇多糖的提取率,本实验初步通过单因素实验和正交实验研究水浴振荡辅助酶法提取双孢蘑菇多糖的工艺条件,研究结果表明:其最佳工艺条件为:料液比为1∶40、振荡频率为40r/min、酶用量为1.2%、pH为6.5、酶解温度为65℃、酶解时间为120min。实验结果表明,双孢蘑菇多糖的提取率可达2.31%。

In order to improve the yield of agaricus bisporus polysaccharide,the initial adoption of single-factor experiments and orthogonal test study of shaking bath-assisted enzymatic extraction of polysaccharides from Agaricus bisporus process conditions were studied.The results showed that the optimum reaction conditions were determined as follows：material/liquid ratio of 1∶40,oscillation frequency was 40r/min,enzyme dosage of 1.2%,pH of 6.5,hydrolysis temperature was 65℃,digestion time was 120min.Experiment results showed the Agaricus bisporus polysaccharides extraction rate can reach 2.31%.