白细胞介素-17及其受体在垂体瘤中的表达及临床意义

Expression of Interleukin-17 and Its Receptor in Pituitary Adenomas and Its Clinical Significance

【目的】检测人垂体腺瘤组织中白细胞介素(IL-17)及其受体IL-17R的表达,并探讨其在垂体腺瘤侵袭性发病机制中的作用。【方法】收集85例人垂体腺瘤组织标本,分成2组:侵袭性垂体腺瘤组45例、非侵袭性垂体腺瘤组40例。采用免疫组化法检测各标本IL-17、IL-17R的表达。随机选取其中50例标本(分成2组:侵袭性组25例、非侵袭性组25例),采用RT-PCR法检测IL-17、IL-17R的mRNA表达水平。分析IL-17、IL-17R的表达与垂体腺瘤侵袭性的关系。【结果】免疫组化结果显示:IL-17、IL-17R在侵袭性垂体腺瘤中的强阳性及总阳性表达率分别38.0%和83.0%、49%和86.7%,而非侵袭性垂体腺瘤中的强阳性及总阳性表达率分别20.0%和52.5%、20.0%和62.5%。IL-17、IL-17R在侵袭性垂体腺瘤中的表达率明显高于在非侵袭性垂体腺瘤中的表达率,统计学分析显示有统计学意义(P<0.05)。RT-PCR结果显示:侵袭性垂体腺瘤中的IL-17、IL-17RmRNA的表达水平明显高于非侵袭性垂体腺瘤,差异有统计学意义(P<0.05)。【结论】IL-17、IL-17R在侵袭性垂体腺瘤组织中的表达明显升高,可能促进了垂体腺瘤侵袭性的发生。

[Objective] The study was designed to investigate the role of IL-17 and IL-17R in the pathogenesis of pituitary adenomas by detecting expression of IL-17 and IL-17R in human pituitary adenomas tissue.[Methods] A total of 85 specimens of human pituitary adenoma tissue were recruited in our study and were divided into 2 groups,including 45 cases of invasive pituitary adenomas and 40 cases of non-invasive pituitary adenomas.Immunohistochemistry was used to detect expression of IL-17,IL-17R in human pituitary adenomas tissue.50 patients were randomly selected and were divided into 2 groups,including 25 cases of invasive pituitary adenomas and 25 cases of non-invasive pituitary adenomas.RT-PCR was used to detect expression levels of IL-17 mRNA,IL-17R mRNA in human pituitary adenomas tissue.To analyze the relationship between IL-17,IL-17R expression and invasiveness of pituitary adenomas.[Results] The results of immunohistochemistry showed that the expression of strong positive rate and the expression of total positive rate of IL-17,IL-17R in invasive pituitary adenomas were 38% and 83%,49% and 86.7% respectively,but that in non-invasive pituitary adenomas were 20% and 57.5%,20% and 62.5% respectively.IL-17,IL-17R expression in invasive pituitary adenomas were significantly higher than that in non-invasive pituitary adenomas.Statistical analysis has statistical significance（P 0.05）.The results of RT-PCR show the levels of IL-17 mRNA,IL-17R mRNA in invasive pituitary adenomas were significantly higher than that in non-invasive pituitary adenomas.Statistical analysis has statistical significance（P 0.05）.[Conclusions] The results of immunohistochemistry and RT-PCR show that the expression of IL-17,IL-17R in invasive pituitary adenomas were significantly higher than that in non-invasive pituitary adenomas.These results indicated that the high expression of IL-17,IL-17R is closely related with invasiveness of pituitary adenoma.