伤寒沙门菌和甲型副伤寒沙门菌分离株的外膜蛋白谱比较

Proteomic Comparison of Outer Membrane Proteins of Salmonella Typhi and Paratyphi A Isolates

目的:比较伤寒沙门菌和甲型副伤寒沙门菌流行菌株的外膜蛋白谱差异。方法:运用二维蛋白电泳方法,对我国伤寒沙门菌株XJ90和甲型副伤寒沙门菌株JX2005-92在实验室通用营养条件下培养提取的外膜蛋白进行分离,比对其差异,对差异蛋白点进行质谱鉴定,对鉴定蛋白点的基因序列也进行比较。结果:菌株XJ90中发现20个特异蛋白点,质谱鉴定出16个;菌株JX2005-92中发现29个特异蛋白点,鉴定出18个。在这些蛋白中,OmpA是数目最多的同种差异蛋白。这些差异蛋白点中的大部分编码基因在2种细菌中序列高度相似或相同。结论:伤寒沙门菌和甲型副伤寒沙门菌基因序列高度相似的外膜蛋白具有不同的修饰形式,提示其不同遗传背景在相同的环境条件下表现出精细的功能差异。

Objective：To reveal the outer membrane protein（OMP） profile difference between Salmonella Typhi and Paratyphi A isolates.Methods：Two-dimensional gel electrophoresis was used to separate the OMP extracts from the Salmonella Typhi strain XJ90 and Salmonella Paratyphi A strain JX2005-92,which were cultures under the common media in laboratory.The OMP profiles of these two strains were compared and the different protein spots were identified with mass spectrometry.Nucleic acid sequences of genes of the identified proteins in these two serotypes were also compared.Results：Twenty specific protein spots of Salmonella Typhi strain XJ90 and 29 proteins of Salmonella Paratyphi A strain JX2005-92 were found,and 16 and 18 spots respective to these two strains were identified with MS.Most of different proteins are coded by their shared genes.Spots identified as OmpA were the most common in the gels.Conclusion：The different OMP spots which share the highly similar gene sequences of these two serotypes may have different protein modification,suggesting elaborate function difference of these shared proteins in these two serotypes.