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MTT实验检测重组人角质细胞生长因子-2诱导不同细胞系增殖的差异 被引量：3

Indication Proliferation Disparity of Different Cell Lines by Recombinant Human KGF-2 Inducation with MTT Assay
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摘要 目的:用MTT法检测重组人角质细胞生长因子-2(rhKGF-2)诱导不同细胞系增殖的差异,建立验证rhKGF-2生物活性的细胞模型。方法:利用载体pBV220-KGF-2原核表达和纯化rhKGF-2,然后用MTT法分别检测rhKGF-2诱导大鼠气管上皮细胞RTE、大鼠小肠上皮细胞IEC-6、小鼠成纤维细胞NIH/3T3和人喉癌细胞Hep-2增殖的能力。结果:rhKGF-2具有强促Hep-2细胞增殖的生物活性,而对RTE细胞、IEC-6细胞、NIH/3T3细胞的诱导增殖作用不明显。结论:在体外,rhKGF-2具有较强的诱导Hep-2细胞增殖的作用。本研究为确立验证rhKGF-2生物活性的细胞模型的选择提供了实验依据。 Objective：To detect the proliferation disparity of different cell lines by recombinant human keratinocyte growth factor-2（rhKGF-2） stimulation with MTT experiment and to establish the cell sample to identify rhKGF-2 bioactivity.Methods：After expression and purification of rhKGF-2 in prokaryotic system,RTE cell,IEC-6 cell,NIH/3T3 cell and Hep-2 cell were prepared to verify the ability of rhKGF-2 to stimulate proliferation of cell lines with MTT experiment.Results：rhKGF-2 had a strong biological activity of promoting Hep-2 cell to proliferate,but to RTE cell,IEC-6 cell and NIH/3T3 cell,its funcation of inducing proliferation was not obvious.Conclusion：rhKGF-2 can induce proliferation of Hep-2 cells in vitro,this study provided a cell model for the establishment of verification rhKGF-2 biological activity.
作者 孙卫国 王芳 刘农乐 丁红梅 李邦印 李少华 邵宁生
机构地区 解放军第 军事医学科学院基础医学研究所
出处 《生物技术通讯》 CAS 2011年第2期225-228,共4页 Letters in Biotechnology
关键词 角质细胞生长因子-2 基因表达 细胞增殖MTT实验 keratinocyte growth factor-2 gene expression MTT cell proliferation test
分类号 Q25 [生物学—细胞生物学] Q78 [生物学—分子生物学]
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参考文献8

  • 1Emoto H,Tagashira S,Mattei M G,et al.Structure and expression of human fibroblast growth factor-10[J].J Biol Chem,1997,272(37):23191-23194.
  • 2Pereira C T,Herndon D N,Rocker R,et al.Liposomal gene transfer of keratinocyte growth factor improves wound heal ing by altering growth factor and collagen expression[J].J Surg Res,2007,139(2):222-228.
  • 3Otte J M,Boser S,Brunke G,et al.Expression of keratinocyte growth factor and its receptor in adaptive changes of ileorectal pouch mucesa[J].Scand J Gastroenterol,2005,40(9):1066-1075.
  • 4Sandbom W J,Sands B E,Wolf D C,et al.Repifermin(keratinocyte growth factor-2)for the treatment of active ulcerative colitis:a randomized double-blind,placebo-controlled,dose-escalation trial[J].Aliment Pharmacoi Ther,2003,17(11):1355-1364.
  • 5Yang H,Antony P A,Wildhaber B E,et al.Intestinal intraepithelial lymphocyte gamma delta-T cell-derived keratinocyte growth factor modulates epithelial growth in the mouse[J].J Immunol,2004,172:4151-4158.
  • 6王园园,王金凤,蔡欣,邹民吉,徐涛,刘深,彭善云,王嘉玺,徐东刚.重组人KGF-2的制备及生物学活性研究[J].军事医学科学院院刊,2008,32(1):34-38. 被引量：11
  • 7方容,胡劲,甄宏韬,崔永华.角质细胞生长因子对喉癌细胞株Hep-2及细胞外调节信号激酶活性及表达的影响[J].临床耳鼻咽喉科杂志,2006,20(24):1132-1134. 被引量：1
  • 8Andresen B T,Rizzo M A,Shome K,et al.The role ofphosphatidic acidin the regulation of the Ras/MEK/Erksignaling cascade[J].FEBS Lett,2002,531:65-68.

二级参考文献21

  • 1Weinstein-Oppenheimer C R,Blalock W L,Steelman L S,et al.The raf signal transduction cascade as a target for chemotherapeutic intervention in growth factor-responsive tumour.Pharmacol Ther,2000,88:229-279.
  • 2Frank L.Protective effect of keratinocyte growth factor against lung abnormalities associated with hyperoxia in prematurely born rats.Biol Neonate,2003,83:263-272.
  • 3Zang X P,Siwak D R,Nguyen T X.KGF-induced motility of breast cancer cells is dependent on Grb2 and Erk1,2.Clin Exp Metastasis,2004,21:437-443.
  • 4Tamaru N,Hishikawa Y,Ejima K,et al.Estrogen receptor-associated exp ression of keratinocyte growth factor and its possible role in the inhibition of apoptosis in human breast cancer.Lab Invest,2004,84:1460-1471.
  • 5Yamayoshi T,Nagayasu T,Matsumoto K.Expression of keratinocyte growth factor fibroblast growth factor-7 and its receptor in human lung cancer:correlation with tumor proliferative activity and patient prognosis.J Pathol,2004,204:110-118.
  • 6Yoshino M,Ishiwata T,Watanabe M,et al Keratinocyte growth factor receptor expression in normal colorectal epithelial cells and differentiated type of colorectal cancer.Oncol Rep,2005,13:247-252.
  • 7Johnson G L,Lapadat R.Mitogen-activated protein kinase pathways mediated by ERK,JNK,and p38 protein kinases.Science,2002,298:1911-1912.
  • 8Andresen B T,Rizzo M A,Shome K,et al.The role of phosphatidic acidin the regulation of the Ras/MEK/Erk signaling cascade.FEBS Lett,2002,531:65-68.
  • 9Webb C P,van Aelst L,Wigler M H,et al.Signaling pathways in Ras-mediated tumorigenicity and metastasis.Proc Natl Acad Sci,1998,95:8773-8778.
  • 10Yamasaki M, Miyake A, Tagashira S, et al. Structure and expression of the rat mRNA encoding a novel member of the fibroblast growth factor family[J]. J Biol Chem, 1996, 271 (27) : 15918 - 15921.

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二级引证文献7

生物技术通讯
2011年 第2期

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