摘要
目的:研究microRNA169b(miRNA169b)启动子不同长度片段的活性及自身调控。方法:用生物信息学方法分析水稻中miRNA169b前体上游1500 bp序列,并从水稻幼苗基因组DNA中扩增miRNA169b前体上游500、1000和1500 bp启动子片段,分别将3个片段克隆至萤光素酶报告基因载体p5XGal4-LUC上。结果:构建了miRNA169b启动子重组载体,序列分析表明与预期结果一致。结论:miRNA169b启动子调控的萤光素酶报告基因表达载体的构建,为研究水稻中miRNA基因自身的转录奠定了基础。
Objective：To research activity and self-regulation of different segments of miRNA169b promoter in rice.Methods：The 1500,1000 and 500 bp DNA segment of rice miRNA169b promoter were analyzed with bioinformatics and amplified by PCR from rice seedlings and cloned to p5XGal4-LUC vectors,then the positive clones were confirmed by restriction enzymes and sequencing.Results：The targeted segments were cloned to Firefly luciferase report vectors p5XGal4-LUC.Conclusion：These results may make an important basis for future studies of miRNA gene promoter activity and regulation of gene expression.
出处
《生物技术通讯》
CAS
2011年第2期234-237,共4页
Letters in Biotechnology
基金
国家自然科学基金(30971704)
