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盐生杜氏藻烯醇酶基因启动子的克隆分析及胁迫转录应答 被引量：2

Cloning of Promoter of the Enolase Gene from Duanliella salina and Its Transcriptional Response to Stress
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摘要 为了解盐生杜氏藻(Dunaliella salina)烯醇酶(Enolase)在渗透耐受中的具体功能,利用基因组步行方法和巢式PCR,从D.salina中克隆了烯醇酶基因DsENO 5’上游约2 000 bp的调控序列,并对其进行序列分析.分析表明,它包含多个与转录调控有关的保守序列(如CAAT-box,TATA-box),富含光﹑干旱及其它胁迫应答元件.利用实时荧光定量PCR的方法,研究了高渗﹑高温以及低温外界胁迫条件下DsENO的转录情况,发现其受高渗强烈抑制,高温显著诱导而低温微弱诱导. With the genome walking method and nested PCR,enolase gene DsENO 5＇upstream regulatory sequence（about 2 000 bp） was cloned from Dunaliella salina,and its sequence analysis was made to investigate the specifi c function of D.salina during osmotic stress tolerance.The result shows that it includes several conserved sequences related to transcriptional regulation,such as CAAT-box,TATA-box,and it is rich in light,drought and other stress responsive elements.With the real-time fluorescence quantitative PCR method,the DsENO transcription under hyperosmotic,high-temperature and low-temperature stress were studied to fi nd that it was strongly inhibited by hyperosmotic stress and significantly induced by high-temperature,but slightly induced by low-temperature.
作者 段静波 李少贺 阮琨 白林含
机构地区 四川大学生命科学学院生物资源与生态环境教育部重点实验室
出处 《应用与环境生物学报》 CAS CSCD 北大核心 2011年第2期202-206,共5页 Chinese Journal of Applied and Environmental Biology
基金 国家自然科学基金项目(Nos.30500006 30970043)资助~~
关键词 盐生杜氏藻 烯醇酶 基因组步行 启动子 实时荧光定量PCR 胁迫应答 Dunaliella salina enolase genome walking promoter real-time fluorescence quantitative PCR response to stress
分类号 Q943.2 [生物学—植物学]
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参考文献19

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二级参考文献11

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共引文献3

同被引文献20

  • 1ZHAOJing-Ya,ZUOKai-Jing,QINJie,TANGKe-Xuan.Molecular Cloning and Characterization of Enolase from Oilseed Rape （Brassica napus）[J].Acta Botanica Sinica,2004,46(10):1226-1233. 被引量：2
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  • 7HOJOUNG L, YAN G, MASARU O, et al. LOS2, a genectic locus required for cold-responsive gene tran- scription encodes a bi-functional enolase[J]. The EM- BO Journal, 2002, 21(11) 2692-2702.
  • 8WOLF A H, SLAYMAN C W, GRADMANN D. Pri- mary structure of the plasma membrane H+-ATPase from the halotolerant alga Dunaliella bioculata [J]. Plant Molecular Biology, 1995, 28(4) : 657-666.
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应用与环境生物学报
2011年 第2期

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